• Product nameAnti-MMP1 antibody [SB12e]
    See all MMP1 primary antibodies
  • Description
    Mouse monoclonal [SB12e] to MMP1
  • Specificityab25483 recognises MMP1. It does not react with MMP2, MMP3 or MMP9.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Fr, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human): MMP1



Our Abpromise guarantee covers the use of ab25483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 5 µg/ml. For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab38924 as Detection.


  • FunctionCleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 27 kDa interstitial collagenase antibody
    • CLG antibody
    • CLGN antibody
    • collagenase, fibroblast antibody
    • collagenase, interstitial antibody
    • Fibroblast collagenase antibody
    • Interstitial collagenase antibody
    • Matrix metallopeptidase 1 (interstitial collagenase) antibody
    • Matrix metalloprotease 1 antibody
    • Matrix Metalloproteinase 1 antibody
    • Matrix metalloproteinase-1 antibody
    • MMP 1 antibody
    • MMP-1 antibody
    • MMP1 antibody
    • MMP1_HUMAN antibody
    • OTTHUMP00000045866 antibody
    see all

Anti-MMP1 antibody [SB12e] images


References for Anti-MMP1 antibody [SB12e] (ab25483)

This product has been referenced in:
  • Lama P  et al. Do intervertebral discs degenerate before they herniate, or after? Bone Joint J 95-B:1127-33 (2013). Read more (PubMed: 23908431) »
  • Fabre V  et al. Tissue inhibitor of matrix-metalloprotease-1 predicts risk of hepatic fibrosis in human Schistosoma japonicum infection. J Infect Dis 203:707-14 (2011). ELISA . Read more (PubMed: 21199883) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

Please find the information below describingour testing discount program:

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it ...

Read More
Application Western blot
Sample Rabbit Tissue lysate - whole (heart)
Loading amount 40 µg
Specification heart
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Oct 13 2010

Thank you for your enquiry. It appears from the information on these antibodies that they have been tested and shown to work in ELISA, but they were all tested using an indirect ELISA only (antigen coated on the plate, then primary and secondary ant...

Read More