Overview

  • Product nameAnti-MMP10 antibodySee all MMP10 primary antibodies ...
  • Description
    Rabbit polyclonal to MMP10
  • SpecificityThis antibody is specific for MMP10, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, etc).
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Dog, Human
  • Immunogen

    Synthetic peptide based on the Hemopexin domain of the human MMP10.

    (Peptide available as ab41076.)

  • EpitopeThis antibody binds to the Hemopexin domain near the carboxyterminus.

Properties

Applications

Our Abpromise guarantee covers the use of ab28206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/1000 - 1/5000. Detects a band of approximately 56 kDa (pro-form, with a predicted molecular weight: 54 kDa) and a 46kDa band (inital active form), using reducing conditions. The final, hemopexin cleaved form is not detected. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCan degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P09238 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Matrix metallopeptidase 10 (stromelysin 2) antibody
      • Matrix metalloprotease 10 antibody
      • Matrix metalloproteinase 10 (stromelysin 2) antibody
      • Matrix Metalloproteinase 10 antibody
      • Matrix Metalloproteinase 10 antibody
      • Matrix metalloproteinase-10 antibody
      • MMP 10 antibody
      • MMP-10 antibody
      • Mmp10 antibody
      • MMP10_HUMAN antibody
      • SL 2 antibody
      • SL-2 antibody
      • SL2 antibody
      • STMY 2 antibody
      • STMY2 antibody
      • STMY2 antibody
      • Stromelysin 2 antibody
      • Stromelysin II antibody
      • Stromelysin II antibody
      • Stromelysin-2 antibody
      • Stromelysin2 antibody
      • StromelysinII antibody
      • Transin 2 antibody
      • Transin-2 antibody
      • Transin2 antibody
      see all

    Anti-MMP10 antibody images

    • All lanes : Anti-MMP10 antibody (ab28206) at 1/1000 dilution

      Lane 1 : MMP-10 at 0.1 µg
      Lane 2 : MMP-10 at 0.01 µg
      Lane 3 : MMP-10 at 0.001 µg
      Lane 4 : MMP-10 at 0.1 µg
      Lane 5 : MMP-10 at 0.01 µg
      Lane 6 : MMP-10 at 0.001 µg


      Predicted band size : 54 kDa
      Observed band size : 46,56 kDa (why is the actual band size different from the predicted?)

    References for Anti-MMP10 antibody (ab28206)

    This product has been referenced in:
    • Doehn U  et al. RSK is a principal effector of the RAS-ERK pathway for eliciting a coordinate promotile/invasive gene program and phenotype in epithelial cells. Mol Cell 35:511-22 (2009). WB ; Human, Dog . Read more (PubMed: 19716794) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"