MMP10 antibody - Hemopexin domain (ab28206)

Product Name 

MMP10 antibody - Hemopexin domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP10 - Hemopexin domain

Immunogen 

Synthetic peptide based on the Hemopexin domain of the human MMP10.

(Peptide available as ab41076.)

Reacts with 
(species key)

Hu

Specificity 

This antibody is specific for MMP10, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, etc).

We have a range of domain specific antibodies for this target. For a full list please see all MMP10 antibodies

Epitope 

This antibody binds to the Hemopexin domain near the carboxyterminus.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. Detects a band of approximately 56 kDa (pro-form, with a predicted molecular weight: 54 kDa) and a 46kDa band (inital active form), using reducing conditions. The final, hemopexin cleaved form is not detected. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

Proteins of the matrix metalloproteinase (MMP) family are zinc containing proteolytic enzymes involved in the breakdown of the extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling. They also play an important role in apoptosis, tumor cell growth, invasion, metastasis, as well as in angiogenesis and wound healing. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.
Most of the MMP's contain a common domain structure, which include a signal sequence, a propeptide, a catalytic domain and a hemopexin-like (Hpx) domain.
MMP10 degrades proteoglycans, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. and fibronectin. It activates procollagenase.
The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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