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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP10 antibody - Hinge region (ab38930)
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Product Name
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MMP10 antibody - Hinge region
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See all MMP10 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MMP10 - Hinge region
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Immunogen
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Synthetic peptide based on the hinge region of the human MMP10 sequence.
(Peptide available as ab41075.)
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Reacts with
(species key)
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Hu
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Specificity
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The antibody binds to the hinge region of MMP10. The antibody binds to Stromelysin 2, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, etc.).
The antibody also recognizes the cleaved active enzyme and will also bind to the non-reduced protein.
We have a range of domain specific antibodies for this target. For a full list please see all MMP10 antibodies
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Tested applications
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IHC-P, WB
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Abreviews
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Application notes
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Recommended dilutions
IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Although the sequence homology for this portion of MMP10 is well conserved, higher concentrations of antibody may be needed for non-human samples. When used against the reduced protein the antibody identifies bands at 56 kDa (the pro-form), as well as the initial active forms (46 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Relevance
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Proteins of the matrix metalloproteinase (MMP) family are zinc containing proteolytic enzymes involved in the breakdown of the extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling. They also play an important role in apoptosis, tumor cell growth, invasion, metastasis, as well as in angiogenesis and wound healing. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.
Most of the MMP's contain a common domain structure, which include a signal sequence, a propeptide, a catalytic domain and a hemopexin-like (Hpx) domain.
MMP10 degrades proteoglycans, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. and fibronectin. It activates procollagenase.
The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
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MMP10 antibody - Hinge region (ab38930) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP10 antibody - Hinge region is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MMP10 antibody - Hinge region images, references, products related to ab38930 and other tools.
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MMP10 antibody - Hinge region images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP10 antibody - Hinge region (ab38930)
Ab38930 staining Human normal trachea. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for MMP10 antibody - Hinge region (ab38930)
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This product has been used in:
Frederick LA et al. Matrix metalloproteinase-10 is a critical effector of protein kinase Ciota-Par6alpha-mediated lung cancer. Oncogene 27:4841-53 (2008). IHC-P. PubMed: 18427549
If you publish research using ab38930 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MMP10
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