MMP10 overexpression 293T lysate (whole cell) (ab94294)

Overview

  • Product nameMMP10 overexpression 293T lysate (whole cell)
  • Description
    MMP10 overexpression lysate
  • General notesab94294 is a 293T cell transfected lysate in which Human MMP10 has been transiently over-expressed using a pCMV-MMP10 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundDomain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Function: Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase. Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

MMP10 overexpression 293T lysate (whole cell) images

  • ab94294 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-MMP10 antibody (ab89638) at 1/500 dilution

    Lane 1 : MMP10 overexpression 293T lysate (whole cell) (ab94294)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for MMP10 overexpression 293T lysate (whole cell) (ab94294)

ab94294 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"