General notesab94294 is a 293T cell transfected lysate in which Human MMP10 has been transiently over-expressed using a pCMV-MMP10 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
BackgroundDomain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Function: Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
Similarity: Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.