• Product nameAnti-MMP11 antibody
    See all MMP11 primary antibodies
  • Description
    Rabbit polyclonal to MMP11
  • Specificityab53143 detects endogenous levels of total MMP11 protein.
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized peptide derived from human MMP11.

  • Positive control
    • WB: A549 cell extract



Our Abpromise guarantee covers the use of ab53143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 55 kDa).
ELISA 1/10000.


  • FunctionMay play an important role in the progression of epithelial malignancies.
  • Tissue specificitySpecifically expressed in stromal cells of breast carcinomas.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrix Metalloproteinase 11 antibody
    • Matrix metalloproteinase-11 antibody
    • MMP-11 antibody
    • Mmp11 antibody
    • MMP11_HUMAN antibody
    • SL 3 antibody
    • SL-3 antibody
    • SL3 antibody
    • ST3 antibody
    • STMY3 antibody
    • Stromelysin 3 antibody
    • Stromelysin III antibody
    • Stromelysin-3 antibody
    see all

Anti-MMP11 antibody images

  • All lanes : Anti-MMP11 antibody (ab53143) at 1/500 dilution

    Lane 1 : A549 cell extract with no peptide
    Lane 2 : A549 cell extract with peptide

    Predicted band size : 55 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Ab53143 staining Human normal placenta. Staining is localised to the extracellular matrix (secreted).
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-MMP11 antibody (ab53143)

This product has been referenced in:
  • Roscilli G  et al. Circulating MMP11 and specific antibody immune response in breast and prostate cancer patients. J Transl Med 12:54 (2014). ELISA ; Human . Read more (PubMed: 24564996) »

See 1 Publication for this product

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