Anti-MMP11 antibody [SL3.05] (Biotin) (ab79470)

Overview

  • Product nameAnti-MMP11 antibody [SL3.05] (Biotin)
    See all MMP11 primary antibodies
  • Description
    Mouse monoclonal [SL3.05] to MMP11 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein corresponding to the active form of Human MMP11

  • Positive control
    • WB: recombinant MMP11 protein IHC-P: Human placenta tissue
  • General notesStable for 24 months when stored at 4°C.

Properties

Applications

Our Abpromise guarantee covers the use of ab79470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
  • Application notesIHC-P: Use at a concentration of 1 - 2 µg/ml. Antigen retrieval is not required.
    IP: Use at 2µg/mg of lysate. Native verified. Use protein G.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects bands of approximately ~60kDa (latent), ~47kDa (active) and ~35kDa (degradation product).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play an important role in the progression of epithelial malignancies.
    • Tissue specificitySpecifically expressed in stromal cells of breast carcinomas.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      The precursor is cleaved by a furin endopeptidase.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Matrix Metalloproteinase 11 antibody
      • Matrix metalloproteinase-11 antibody
      • MMP-11 antibody
      • Mmp11 antibody
      • MMP11_HUMAN antibody
      • SL 3 antibody
      • SL-3 antibody
      • SL3 antibody
      • ST3 antibody
      • STMY3 antibody
      • Stromelysin 3 antibody
      • Stromelysin III antibody
      • Stromelysin-3 antibody
      see all

    Anti-MMP11 antibody [SL3.05] (Biotin) images

    • Immunohistochemistry analysis of formalin/fixed, paraffin-embedded Human placenta tissue, using the unconjugated version of ab79470, at 2.0µg/ml, with a peroxidase-conjugated secondary antibody and an AEC chromogen. Note cytoplasmic staining of trophoblasts.

    References for Anti-MMP11 antibody [SL3.05] (Biotin) (ab79470)

    ab79470 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab79470.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"