The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Use at a concentration of 1 - 2 µg/ml. Antigen retrieval is not required.
IP: Use at 2µg/mg of lysate. Native verified. Use protein G.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects bands of approximately ~60kDa (latent), ~47kDa (active) and ~35kDa (degradation product).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play an important role in the progression of epithelial malignancies.
Tissue specificitySpecifically expressed in stromal cells of breast carcinomas.
Sequence similaritiesBelongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modificationsThe precursor is cleaved by a furin endopeptidase.
Cellular localizationSecreted > extracellular space > extracellular matrix.
Immunohistochemistry analysis of formalin/fixed, paraffin-embedded Human placenta tissue, using the unconjugated version of ab79470, at 2.0µg/ml, with a peroxidase-conjugated secondary antibody and an AEC chromogen.
Note cytoplasmic staining of trophoblasts.
References for Anti-MMP11 antibody [SL3.05] (Biotin) (ab79470)
has not yet been referenced specifically in any publications.
Publishing research using ab79470? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"