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Product name
MMP11 protein (Human)
See all MMP11 products (5) ...
Protein description
Full length Human MMP11 protein (purified from stimulated human fibroblasts) and supplied as a mixture of zymogen and processed (cleaved)forms.
Expression host
Native
Properties
Purity
> 95% by SDS-PAGE
Purification notes
This protein is free of contaminating MMPs or TIMPs.
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 0.02% Brij 35, 5mM Calcium chloride, 100mM Sodium chloride, 10mM Tris HCl, pH 7.4
Concentration
Concentration information loading...
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 60,50 kDa.60 kDa (Zymogen) and 50 kDa (processed form). Dilution optimised using Chromogenic detection.
ELISA: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
May play an important role in the progression of epithelial malignancies.
Tissue specificity
Specifically expressed in stromal cells of breast carcinomas.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP24347
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for MMP11 protein (Human) (ab39310)
ab39310 has not yet been referenced specifically in any publications.
Publishing research using ab39310? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"