Overview

  • Product nameAnti-MMP12 antibody
    See all MMP12 primary antibodies
  • Description
    Rabbit polyclonal to MMP12
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human MMP12.

  • Positive control
    • This antibody gave a positive signal in both A549 and MDA MB 361 whole cell lysates and human lung FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab113514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 54 kDa).

Target

  • FunctionMay be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
  • Tissue specificityFound in alveolar macrophages but not in peripheral blood monocytes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • FormThe presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
  • Alternative names
    • EC 3.4.24.65 antibody
    • HME antibody
    • Macrophage elastase antibody
    • Macrophage metalloelastase antibody
    • Macrophage metaloelastase antibody
    • Matrix metallopeptidase 12 (macrophage elastase) antibody
    • Matrix metalloprotease 12 antibody
    • Matrix metalloproteinase-12 antibody
    • ME antibody
    • MGC138506 antibody
    • MME antibody
    • MMP 12 antibody
    • MMP-12 antibody
    • Mmp12 antibody
    • MMP12_HUMAN antibody
    see all

Anti-MMP12 antibody images

  • All lanes : Anti-MMP12 antibody (ab113514) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • IHC image of ab113514 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113514, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-MMP12 antibody (ab113514)

ab113514 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab113514.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"