Overview

  • Product name
  • Description
    Rabbit polyclonal to MMP12
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 203-441 of Human MMP12 (NP_002417).

  • Positive control
    • 293T whole cell lysate; Gastric CAN 87 xenograft tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: A549

Properties

Applications

Our Abpromise guarantee covers the use of ab137444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
  • Tissue specificity
    Found in alveolar macrophages but not in peripheral blood monocytes.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Form
    The presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
  • Alternative names
    • EC 3.4.24.65 antibody
    • HME antibody
    • Macrophage elastase antibody
    • Macrophage metalloelastase antibody
    • Macrophage metaloelastase antibody
    • Matrix metallopeptidase 12 (macrophage elastase) antibody
    • Matrix metalloprotease 12 antibody
    • Matrix metalloproteinase-12 antibody
    • ME antibody
    • MGC138506 antibody
    • MME antibody
    • MMP 12 antibody
    • MMP-12 antibody
    • Mmp12 antibody
    • MMP12_HUMAN antibody
    see all

Images

  • ICC/IF image of ab137444 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab137444 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-MMP12 antibody (ab137444) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 54 kDa
    7.5% SDS PAGE
  • Immunohistochemical analysis of parafin embedded Gastric CAN 87 xenograft tissue labelling MMP12 with ab137444 at 1/100 dilution.

References

ab137444 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Other
Sample
Human Tissue sections (gingiva)
Specification
gingiva
Permeabilization
No
Fixative
Formaldehyde
Username

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Verified customer

Submitted Dec 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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