Overview

  • Product nameAnti-MMP12 antibody [EP1261Y]
    See all MMP12 primary antibodies
  • Description
    Rabbit monoclonal [EP1261Y] to MMP12
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MMP12 aa 450 to the C-terminus (C terminal).
    Database link: P39900

  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Alternative versions available:

    Anti-MMP12 antibody (Alexa Fluor® 488) [EP1261Y] (ab200409)

    Anti-MMP12 antibody (Alexa Fluor® 647) [EP1261Y] (ab200410)

Properties

Applications

Our Abpromise guarantee covers the use of ab52897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP 1/20.

For unpurified use at 1/30.

  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctionMay be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
    • Tissue specificityFound in alveolar macrophages but not in peripheral blood monocytes.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • FormThe presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
    • Alternative names
      • EC 3.4.24.65 antibody
      • HME antibody
      • Macrophage elastase antibody
      • Macrophage metalloelastase antibody
      • Macrophage metaloelastase antibody
      • Matrix metallopeptidase 12 (macrophage elastase) antibody
      • Matrix metalloprotease 12 antibody
      • Matrix metalloproteinase-12 antibody
      • ME antibody
      • MGC138506 antibody
      • MME antibody
      • MMP 12 antibody
      • MMP-12 antibody
      • Mmp12 antibody
      • MMP12_HUMAN antibody
      see all

    Anti-MMP12 antibody [EP1261Y] images

    • Anti-MMP12 antibody [EP1261Y] (ab52897) at 1/5000 dilution (purified) + RAW264.7 spleen lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 54 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-MMP12 antibody [EP1261Y] (ab52897) at 1/2000 dilution (purified) + Rat spleen lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 54 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-MMP12 antibody [EP1261Y] (ab52897) at 1/2000 dilution (purified)

      Lane 1 : HT-1080 cell lysate
      Lane 2 : WI-38 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 54 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.



    • Predicted band size : 54 kDa

      Based on Western blot, unpurified ab52897 recognizes both pro and active MMP. The propeptide is 54 kDa, and 45 kDa and 22 kDa are active forms. The primary was used at 1:100 to detect recombinant MMP12 protein, but can be diluted more to decrease the intensity of the bands.

    • ab52897 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in HeLa whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP secondary antibody (ab131366) (1/1,500) was used as the secondary antibody. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References for Anti-MMP12 antibody [EP1261Y] (ab52897)

    This product has been referenced in:
    • Moon HJ  et al. Effects of secreted factors in culture medium of annulus fibrosus cells on microvascular endothelial cells: elucidating the possible pathomechanisms of matrix degradation and nerve in-growth in disc degeneration. Osteoarthritis Cartilage 22:344-54 (2014). WB ; Human . Read more (PubMed: 24361793) »
    • Kim B  et al. Retinal MMP-12, MMP-13, TIMP-1, and TIMP-2 expression in murine experimental retinal detachment. Invest Ophthalmol Vis Sci 55:2031-40 (2014). WB ; Mouse . Read more (PubMed: 24526442) »

    See all 11 Publications for this product

    Product Wall

    I can confirm that both ab52897 and ab124964 are rabbit IgG monoclonals.

    We have several suitable isotype controls available including the following. These have both been tested in IHC-P. I can recommend to review the online datasheets for f...

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    Thank you for contacting us and your interest in our products. As discussed over the phone, I have checked the concentration of the antibodies and lot numbers you provided. The concentrations are as follows:

    ab52897, GR5286-6, 0.165 mg/ml;
    Read More

    Merci de nous avoir contactés.

    ab52897 est un monoclonal de lapin. Il n'y a pas de sous-classe spécifique d'IgG chez le lapin comme c'est le cas chez la souris ou l'humain par exemple. Tous les monoclonaux de lapin sont donc simp...

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    Thank you very much for your call today and for letting us know about the trouble with this antibody.

    As we discussed, I'm sending a free of charge vial of ab66157 on the order *** which should arrive tomorrow.

    Please keep me update...

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    Thank you for contacting us.

    The concentration of lot GR5286-7 is 0.165 mg/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products...

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    Vielen Dank für Ihre weitere Anfrage. Wir haben diesen Antikörper bisher noch nicht Kryoschnitten getestet, daher wissen wir leider nicht, ob dies funktioniert. Da der Antikörper allerdings in der ICC und dem FACS funktioniert, sollte es meiner M...

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    Laut dem Datenblatt sollte eine Antigen Demaskierung durchgeführt werden. Unser Standardprotokoll sieht dazu eine Mikrowellenbehandlung von 20 in einer TRSI/EDTA- Lösung von pH8 vor, falls Sie allerdings lieber mit Citrat-Lösung pH6 arbeiten, soll...

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    Thank you for your inquiry. Unfortunately we do not carry the blocking peptide for this product and it is not commercially available. I am sorry that this information could not be more helpful. Please contact us with any other questions.

    DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab52897 in IHC-Fr in mouse tissue. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please subm...

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    Thank you for your enquiry. The antigen retrieval method used for this product was pressure cooker method. Please find the details of the protocol below. Pressure cooker antigen retrieval protocol. Solution: Tris-EDTA Buffer (10mM Tris Base, 1...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"