Anti-MMP12 antibody [MM0025-7G20] (ab56305)

Overview

  • Product nameAnti-MMP12 antibody [MM0025-7G20]
    See all MMP12 primary antibodies
  • Description
    Mouse monoclonal [MM0025-7G20] to MMP12
  • SpecificityThis antibody recognizes human MMP12 in western blot without cross reactivity with other human MMPs.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human MMP12

Properties

Applications

Our Abpromise guarantee covers the use of ab56305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/300.
WB Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.

Target

  • FunctionMay be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
  • Tissue specificityFound in alveolar macrophages but not in peripheral blood monocytes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • FormThe presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
  • Alternative names
    • EC 3.4.24.65 antibody
    • HME antibody
    • Macrophage elastase antibody
    • Macrophage metalloelastase antibody
    • Macrophage metaloelastase antibody
    • Matrix metallopeptidase 12 (macrophage elastase) antibody
    • Matrix metalloprotease 12 antibody
    • Matrix metalloproteinase-12 antibody
    • ME antibody
    • MGC138506 antibody
    • MME antibody
    • MMP 12 antibody
    • MMP-12 antibody
    • Mmp12 antibody
    • MMP12_HUMAN antibody
    see all

Anti-MMP12 antibody [MM0025-7G20] images

  • ab56305 at 1/300 dilution, staining MMP12 in human placenta tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

References for Anti-MMP12 antibody [MM0025-7G20] (ab56305)

ab56305 has not yet been referenced specifically in any publications.

Product Wall

Considering that you would like to perform IHC on rabbit specimen, I agree that it is advisable to avoid antibodies generated in rabbit. Therefore, although the MMP12 antibody ab56305 shows a rather low sequence similarity across the full-length protei...

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Thank you very much for your interest in our antibodies. The immunogen and epitope sequences, respectively, show the following similarities against rabbit: 1) ab70191: 93% 2) ab56777: 96% 3) ab56305: 72% 4) ab6328: 100% Due to this high hom...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"