Overview

  • Product nameAnti-MMP13 antibodySee all MMP13 primary antibodies ...
  • Description
    Rabbit polyclonal to MMP13
  • Specificityab39012 recognizes the latent proenzyme, at 60 Kd, as well as the active form at 48 Kd, and intermediate activation forms. It does not cross react with the other MMP family members.
  • Tested applicationsIHC-Fr, IHC-P, WB, ICC/IF, Sandwich ELISA, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Synthetic peptide based on the hinge region of human MMP13.

    (Peptide available as ab44853.)

  • Epitopeab39012 recognizes the Hinge region of MMP13.
  • Positive control
    • Cell media from human chondrosarcoma (IL1 beta treated). Mouse endochondral precursor (rib cartilage) tissue. Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab39012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr 1/200.
IHC-P Use at an assay dependent dilution.
WB 1/3000 - 1/6000. Predicted molecular weight: 54 kDa.Can be blocked with MMP13 peptide (ab44853). 1/3000, when using colorimetric substrates such as BCIP/NBT - 1/6000, when using chemiluminescent substrates. Dilution optimised using Chromogenic detection. When used against the reduced protein identifies a band at 60 Kd. Note: The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting.
ICC/IF Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab77949 as Capture.
Flow Cyt Use at an assay dependent concentration.

Target

  • FunctionDegrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
  • Tissue specificitySeems to be specific to breast carcinomas.
  • Involvement in diseaseDefects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P45452 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CLG 3 antibody
    • CLG3 antibody
    • CLG3 antibody
    • Collagenase 3 antibody
    • Collagenase 3 antibody
    • Collagenase3 antibody
    • MANDP1 antibody
    • Matrix metallopeptidase 13 (collagenase 3) antibody
    • Matrix Metalloproteinase 13 antibody
    • Matrix Metalloproteinase 13 antibody
    • Matrix metalloproteinase-13 antibody
    • MMP 13 antibody
    • MMP-13 antibody
    • Mmp13 antibody
    • MMP13_HUMAN antibody
    see all

Anti-MMP13 antibody images

  • All lanes : Anti-MMP13 antibody (ab39012) at 1/3000 dilution

    Lane 1 : cell media from human chondrosarcoma (untreated).
    Lane 2 : cell media from human chondrosarcoma (IL1 beta treated).


    Predicted band size : 54 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    The loading amount is 15 ul/lane, and the condrosarcoma media was concentrated 40x.
  • ab39012 staining MMP13 in Mouse endochondral precursor (rib cartilage) tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 10% serum for 1 hour at 19°C. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 647-conjugated Donkey anti-Rabbit polyclonal (ab150075) was used as the secondary antibody, diluted 1/200. DAPI was used for the blue counterstain.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue (ab3950), staining MMP13 with ab39012.

    Tissue was fixed with formaldehyde and blocked with 1% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 12 hours at 4&degC. A biotinylated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

  • Standard curve for MMP13; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [181-15A12] to MMP13 (ab77949) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP13 - Hinge region (ab39012) at 0.5µg/ml.

References for Anti-MMP13 antibody (ab39012)

This product has been referenced in:
  • Moon HJ  et al. Effects of secreted factors in culture medium of annulus fibrosus cells on microvascular endothelial cells: elucidating the possible pathomechanisms of matrix degradation and nerve in-growth in disc degeneration. Osteoarthritis Cartilage 22:344-54 (2014). WB ; Human . Read more (PubMed: 24361793) »
  • Ushijima T  et al. CCAAT/enhancer-binding protein ß regulates the repression of type II collagen expression during the differentiation from proliferative to hypertrophic chondrocytes. J Biol Chem 289:2852-63 (2014). IHC-P ; Mouse . Read more (PubMed: 24344131) »

See all 10 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton in PBS
Sample Human Cell (MCF-7 (invasive ductal carcinoma line))
Specification MCF-7 (invasive ductal carcinoma line)
Gating Strategy isotype control (black line in histogram)
Preparation Cell harvesting/tissue preparation method: accutase and accumax dissociation
Sample buffer: PBS 7.4 + 10% FBS for washing
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Submitted Mar 04 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (gingiva)
Specification gingiva
Permeabilization No
Fixative Formaldehyde
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Submitted Dec 16 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA - Buffer ph 9,0
Sample Human Tissue sections (Breast Cancer)
Specification Breast Cancer
Permeabilization Yes - Wash-Buffer from Dako with Tween
Fixative Paraformaldehyde
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Submitted Aug 09 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (rat vascular smooth muscle cells)
Specification rat vascular smooth muscle cells
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
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Submitted Feb 06 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (colon carcinoma)
Specification colon carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0)
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
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Submitted Feb 05 2013

Thank you for contacting us and your interest in our products. As discussed over the phone, I have checked the concentration of the antibodies and lot numbers you provided. The concentrations are as follows:

ab52897, GR5286-6, 0.165 mg/ml;
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Thank you for your inquiry.

Are the gel percentages, compositions and running conditions (time, buffer, etc)the same for both WBs? Are you casting your own gels or buying them pre-made? Have you run a WB with both the cell lysates and medium ...

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Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I am so...

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Application Western blot
Sample Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
Loading amount 100000 cells
Specification rat vascular smooth muscle cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jul 31 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon Carcinoma)
Specification Colon Carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization No
Blocking step (agent) for 5 minute(s) · Concentration: .25% · Temperature: 25°C
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Submitted Nov 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"