Synthetic peptide based on the hinge region of human MMP13.
(Peptide available as ab44853.)
Our Abpromise guarantee covers the use of ab39012 in the following tested applications.
|IHC-P||Use at an assay dependent dilution.|
|WB||1/3000 - 1/6000. Predicted molecular weight: 54 kDa.Can be blocked with MMP13 peptide (ab44853). 1/3000, when using colorimetric substrates such as BCIP/NBT - 1/6000, when using chemiluminescent substrates. Dilution optimised using Chromogenic detection. When used against the reduced protein identifies a band at 60 Kd. Note: The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting.|
|ICC/IF||Use at an assay dependent concentration.|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab77949 as Capture.|
|Flow Cyt||Use at an assay dependent concentration.|
ab39012 staining MMP13 in Mouse endochondral precursor (rib cartilage) tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 10% serum for 1 hour at 19°C. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 647-conjugated Donkey anti-Rabbit polyclonal (ab150075) was used as the secondary antibody, diluted 1/200. DAPI was used for the blue counterstain.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue (ab3950), staining MMP13 with ab39012.
Tissue was fixed with formaldehyde and blocked with 1% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 12 hours at 4°C. A biotinylated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.