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Synthetic peptide based on the hinge region of human MMP13.
(Peptide available as ab44853.)
The Abreview shows successful use of this antibody with frozen sections (IHC-Fr), we do not test it in-house hence we are unable to guarantee its use in this application. For more details contact Scientific Support team.
Our Abpromise guarantee covers the use of ab39012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/3000 - 1/6000. Predicted molecular weight: 54 kDa.Can be blocked with MMP13 peptide (ab44853). 1/3000, when using colorimetric substrates such as BCIP/NBT - 1/6000, when using chemiluminescent substrates. Dilution optimised using Chromogenic detection. When used against the reduced protein identifies a band at 60 Kd. Note: The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting.|
|ICC/IF||Use at an assay dependent concentration.|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [181-15A12] to MMP13 (ab77949). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab77949 as Capture.|
|Flow Cyt||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue (ab3950), staining MMP13 with ab39012.
Tissue was fixed with formaldehyde and blocked with 1% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 12 hours at 4°C. A biotinylated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.
ab39012 staining MMP13 in mouse cartilage and meniscus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with ainc buffered formalin and blocked with 10% serum for 1 hour at 27°C. Samples were incubated with primary antibody (1/400 in TBST) for 15 hours at 4°C. A steptavidin-conjugated goat anti-rabbit IgG polyclonal (biotinylated, 1/200) was used as the secondary antibody.
ab39012 staining MMP13 in Mouse endochondral precursor (rib cartilage) tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 10% serum for 1 hour at 19°C. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4°C. A donkey anti-rabbit Alexa Fluor® 647 polyclonal (ab150075) was used as the secondary antibody, diluted 1/200. DAPI was used for the blue counterstain.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"