Overview

  • Product name
  • Description
    Rabbit polyclonal to MMP13
  • Specificity
    ab39012 recognizes the latent proenzyme, at 60 Kd, as well as the active form at 48 Kd, and intermediate activation forms. It does not cross react with the other MMP family members. ab39012 recognizes the Hinge region of MMP13.
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Sandwich ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Synthetic peptide based on the hinge region of human MMP13.

    (Peptide available as ab44853.)

  • Positive control
    • Cell media from human chondrosarcoma (IL1 beta treated). Mouse endochondral precursor (rib cartilage) tissue. Human colon carcinoma tissue.
  • General notes

    The Abreview shows successful use of this antibody with frozen sections (IHC-Fr), we do not test it in-house hence we are unable to guarantee its use in this application. For more details contact Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab39012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/3000 - 1/6000. Predicted molecular weight: 54 kDa. 1/3000, when using colorimetric substrates such as BCIP/NBT - 1/6000, when using chemiluminescent substrates. Dilution optimised using Chromogenic detection. When used against the reduced protein identifies a band at 60 Kd. Note: The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting.
ICC/IF Use at an assay dependent concentration. PubMed: 24056368
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab77949 as Capture.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
  • Tissue specificity
    Seems to be specific to breast carcinomas.
  • Involvement in disease
    Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLG 3 antibody
    • CLG3 antibody
    • Collagenase 3 antibody
    • Collagenase3 antibody
    • MANDP1 antibody
    • Matrix metallopeptidase 13 (collagenase 3) antibody
    • Matrix Metalloproteinase 13 antibody
    • Matrix metalloproteinase-13 antibody
    • MMP 13 antibody
    • MMP-13 antibody
    • Mmp13 antibody
    • MMP13_HUMAN antibody
    see all

Anti-MMP13 antibody images

  • All lanes : Anti-MMP13 antibody (ab39012) at 1/3000 dilution

    Lane 1 : cell media from human chondrosarcoma (untreated).
    Lane 2 : cell media from human chondrosarcoma (IL1 beta treated).


    Predicted band size : 54 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    The loading amount is 15 ul/lane, and the condrosarcoma media was concentrated 40x.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue (ab3950), staining MMP13 with ab39012.

    Tissue was fixed with formaldehyde and blocked with 1% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 12 hours at 4&degC. A biotinylated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

  • ab39012 staining MMP13 in Mouse endochondral precursor (rib cartilage) tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 10% serum for 1 hour at 19°C. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4°C. A donkey anti-rabbit Alexa Fluor® 647 polyclonal (ab150075) was used as the secondary antibody, diluted 1/200. DAPI was used for the blue counterstain.

    See Abreview

  • ab39012 staining MMP13 in mouse cartilage and meniscus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with ainc buffered formalin and blocked with 10% serum for 1 hour at 27°C. Samples were incubated with primary antibody (1/400 in TBST) for 15 hours at 4°C. A steptavidin-conjugated goat anti-rabbit IgG polyclonal (biotinylated, 1/200) was used as the secondary antibody.

    See Abreview

  • Standard curve for MMP13; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [181-15A12] to MMP13 (ab77949) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP13 - Hinge region (ab39012) at 0.5µg/ml.

References for Anti-MMP13 antibody (ab39012)

This product has been referenced in:
  • McKay TB  et al. Acute hypoxia influences collagen and matrix metalloproteinase expression by human keratoconus cells in vitro. PLoS One 12:e0176017 (2017). WB ; Human . Read more (PubMed: 28426715) »
  • Hu J  et al. BMSC paracrine activity attenuates interleukin-1ß-induced inflammation and apoptosis in rat AF cells via inhibiting relative NF-?B signaling and the mitochondrial pathway. Am J Transl Res 9:79-89 (2017). WB ; Rat . Read more (PubMed: 28123635) »

See all 26 Publications for this product

Product Wall

Thank you for your inquiry.

Are the gel percentages, compositions and running conditions (time, buffer, etc)the same for both WBs? Are you casting your own gels or buying them pre-made? Have you run a WB with both the cell lysates and medium ...

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Thank you for contacting us and your interest in our products. As discussed over the phone, I have checked the concentration of the antibodies and lot numbers you provided. The concentrations are as follows:

ab52897, GR5286-6, 0.165 mg/ml;
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Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I am so...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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