• Product nameAnti-MMP13 antibody
    See all MMP13 primary antibodies
  • Description
    Rabbit polyclonal to MMP13
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Human recombinant MMP13 protein.

  • Positive control
    • Conditioned, serum-free medium from (TPA-treated) rat CCL-4 cells. Placenta tissue.



Our Abpromise guarantee covers the use of ab75606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 - 5 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 10 - 20 µg/ml.


  • FunctionDegrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
  • Tissue specificitySeems to be specific to breast carcinomas.
  • Involvement in diseaseDefects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLG 3 antibody
    • CLG3 antibody
    • Collagenase 3 antibody
    • Collagenase3 antibody
    • MANDP1 antibody
    • Matrix metallopeptidase 13 (collagenase 3) antibody
    • Matrix Metalloproteinase 13 antibody
    • Matrix metalloproteinase-13 antibody
    • MMP 13 antibody
    • MMP-13 antibody
    • Mmp13 antibody
    • MMP13_HUMAN antibody
    see all

Anti-MMP13 antibody images

  • Anti-MMP13 antibody (ab75606) at 5 µg/ml + Conditioned, serum-free medium from (TPA-treated) rat CCL-4 cells

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • ab75606 staining MMP13 in Rat shoulder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked witha vector kit for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in antibody diluent) for 16 hours at 4°C. A undiluted Biotin-conjugated polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-MMP13 antibody (ab75606)

This product has been referenced in:
  • Liang W  et al. Observing the development of the temporomandibular joint in embryonic and post-natal mice using various staining methods. Exp Ther Med 11:481-489 (2016). IHC-P ; Mouse . Read more (PubMed: 26893634) »
  • Bartmann J  et al. A novel role of MMP-13 for murine DC function: its inhibition dampens T-cell activation. Int Immunol 28:473-487 (2016). Read more (PubMed: 26921214) »

See all 15 Publications for this product

Product Wall

This antibody should detect the propeptide and the active form of MMP13. The pro form should be around 60KDa and the active form should be around 48kDa.

Thank you for submitting an Abreview of ab75606. As you may have noticed, your review has now been published on our website.
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Application Western blot
Sample Mouse Tissue lysate - whole (Cerebellum Tumor Cell)
Loading amount 30 µg
Specification Cerebellum Tumor Cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

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Submitted Oct 31 2012

Thank you for confirming those details.
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Expiration date: 19-08-2012

I am very pleased to hear you would like to accept our offer and test ab75606 in Hamster. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before t...

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