Anti-MMP13 antibody (ab9128)

Overview

• Product name
  Anti-MMP13 antibody
  See all MMP13 primary antibodies
• Description
  Rabbit polyclonal to MMP13
• Specificity
  Based on Western blotting, the antibody reacts with human procollagenase-3. It does not react to a significant extent with human gelatinase A, gelatinase B, MT1-MMP, and the catalytic domains of MT2-MMP, MT3-MMP, MT4-MMP and MT5-MMP.
• Tested applications
  Suitable for: WB, ELISAmore details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  This polyclonal antibody was raised against recombinant human procollagenase-3 expressed in insect cells

• General notes
  MMP stands for "Matrix Metalloproteinase"

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  PBS (20 mM Na-phosphate, pH 7.5, 150 mM NaCl, 0.01 % thimerosal)
• Concentration information loading...
• Purity
  Protein A purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Light chain type
  unknown
• Research areas
  • Cardiovascular
  • Angiogenesis
  • Adhesion / ECM
  • Matrix Metalloproteinases
  • MMP

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Enzymes
  • MMP

  • Cancer
  • Invasion/microenvironment
  • Angiogenesis
  • ECM enzymes
  • MMPs

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • MMPs

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Metalloprotease
  • MMPs

  • Cancer
  • Tumor biomarkers
  • Enzymes
  • MMPs

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Tumor biomarkers ELISA kits

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
• Tissue specificity
  Seems to be specific to breast carcinomas.
• Involvement in disease
  Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
  Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
• Sequence similarities
  Belongs to the peptidase M10A family.
  Contains 4 hemopexin-like domains.
• Domain
  The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
• Cellular localization
  Secreted > extracellular space > extracellular matrix.

• Target information above from: UniProt accession P45452 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 4322 Human
  • Omim: 600108 Human
  • SwissProt: P45452 Human
  • Unigene: 2936 Human
• Alternative names
  • CLG 3 antibody
  • CLG3 antibody
  • Collagenase 3 antibody

  • Collagenase3 antibody
  • MANDP1 antibody
  • Matrix metallopeptidase 13 (collagenase 3) antibody
  • Matrix Metalloproteinase 13 antibody
  • Matrix metalloproteinase-13 antibody
  • MMP 13 antibody
  • MMP-13 antibody
  • Mmp13 antibody
  • MMP13_HUMAN antibody

  see all

Protocols

• Western blot protocols