Overview

  • Product name
  • Description
    Rabbit polyclonal to MMP13
  • Specificity
    Based on Western blotting, the antibody reacts with human procollagenase-3. It does not react to a significant extent with human gelatinase A, gelatinase B, MT1-MMP, and the catalytic domains of MT2-MMP, MT3-MMP, MT4-MMP and MT5-MMP.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This polyclonal antibody was raised against recombinant human procollagenase-3 expressed in insect cells

  • General notes
    MMP stands for "Matrix Metalloproteinase"

Properties

Applications

Our Abpromise guarantee covers the use of ab9128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/2500 (PMID 18562219).
    WB: 1/2500 - 1/8000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
    • Tissue specificity
      Seems to be specific to breast carcinomas.
    • Involvement in disease
      Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
      Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
    • Sequence similarities
      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • Domain
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CLG 3 antibody
      • CLG3 antibody
      • Collagenase 3 antibody
      • Collagenase3 antibody
      • MANDP1 antibody
      • Matrix metallopeptidase 13 (collagenase 3) antibody
      • Matrix Metalloproteinase 13 antibody
      • Matrix metalloproteinase-13 antibody
      • MMP 13 antibody
      • MMP-13 antibody
      • Mmp13 antibody
      • MMP13_HUMAN antibody
      see all

    References

    This product has been referenced in:
    • Jones SW  et al. Mitogen-activated protein kinase-activated protein kinase 2 (MK2) modulates key biological pathways associated with OA disease pathology. Osteoarthritis Cartilage 17:124-31 (2009). ELISA ; Human . Read more (PubMed: 18562219) »
    • Jones SW  et al. The identification of differentially expressed microRNA in osteoarthritic tissue that modulate the production of TNF-alpha and MMP13. Osteoarthritis Cartilage 17:464-72 (2009). Read more (PubMed: 19008124) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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