The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.
FunctionDegrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
Tissue specificitySeems to be specific to breast carcinomas.
Involvement in diseaseDefects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age. Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
Sequence similaritiesBelongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localizationSecreted > extracellular space > extracellular matrix.
Anti-MMP13 antibody [EP1263Y] (ab51072) at 1/500 dilution + HeLa cell lysate at 10 µg
Secondary Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size : 54 kDa Observed band size : 54 kDa
References for Anti-MMP13 antibody [EP1263Y] (ab51072)
This product has been referenced in:
Li CM et al. CCL17 Induces Trophoblast Migration and Invasion by Regulating Matrix Metalloproteinase and Integrin Expression in Human First-Trimester Placenta. Reprod SciN/A:N/A (2014).
Read more (PubMed: 24401476) »
Ryu JH et al. Hypoxia-inducible factor-2a is an essential catabolic regulator of inflammatory rheumatoid arthritis. PLoS Biol12:e1001881 (2014).
Read more (PubMed: 24914685) »