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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP13 antibody - Hinge region
See all MMP13 products (18) ...
Description
Rabbit polyclonal to MMP13 - Hinge region
Specificity
ab39012 recognizes the latent proenzyme, at 60 Kd, as well as the active form at 48 Kd, and intermediate activation forms. It does not cross react with the other MMP family members.
Tested applications
IHC-Fr, IHC-P, WB, Sandwich ELISAmore details
Cross reactivity
Reacts with
Mouse, Dog, Human
Immunogen
Synthetic peptide based on the hinge region of human MMP13.
(Peptide available as ab44853.)
Epitope
ab39012 recognizes the Hinge region of MMP13.
Positive control
cell media from human chondrosarcoma (IL1 beta treated).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20ºC.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/200
IHC-P: Use at an assay dependent dilution. (PubMed: 15066996)
WB: 1/3000 - 1/6000.Predicted molecular weight: 54 kDa.(1/3000, when using colorimetric substrates such as BCIP/NBT - 1/6000, when using chemiluminescent substrates. Dilution optimised using Chromogenic detection. When used against the reduced protein identifies a band at 60 Kd. Note: The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting.)
sELISA: Use a concentration of 0.5 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal [181-15A12] to MMP13 (ab77949). (For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab77949 as Capture.)
Target
Function
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
Tissue specificity
Seems to be specific to breast carcinomas.
Involvement in disease
Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP45452
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CLG 3 antibody
- CLG3 antibody
- CLG3 antibody
see all
Database links
- Entrez Gene: 403763 Dog
- Entrez Gene: 4322 Human
- Entrez Gene: 17386 Mouse
- Omim: 600108 Human
- SwissProt: P45452 Human
- SwissProt: Q6NWN6 Human
- SwissProt: P33435 Mouse
- SwissProt: Q3U2N6 Mouse
see all
Anti-MMP13 antibody - Hinge region images:
Sandwich ELISA - MMP13 antibody - Hinge region (ab39012)
Standard curve for MMP13; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [181-15A12] to MMP13 (ab77949) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP13 - Hinge region (ab39012) at 0.5µg/ml.
Western blot - MMP13 antibody - Hinge region (ab39012)
All lanes : Anti-MMP13 antibody - Hinge region (ab39012) at 1/3000 dilution
Lane 1 : cell media from human chondrosarcoma (untreated).
Lane 2 : cell media from human chondrosarcoma (IL1 beta treated).
Predicted band size : 54 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
The loading amount is 15 ul/lane, and the condrosarcoma media was concentrated 40x.
Immunohistochemistry (Frozen sections) - MMP13 antibody - Hinge region (ab39012)
ab39012 staining MMP13 in Mouse endochondral precursor (rib cartilage) tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 10% serum for 1 hour at 19°C. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 647-conjugated Donkey anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200. DAPI was used for the blue counterstain.
This image is courtesy of an anonymous Abreview.
References for Anti-MMP13 antibody - Hinge region (ab39012)
This product has been referenced in:
- Dranse HJet al. Genetic deletion of Cyp26b1 negatively impacts limb skeletogenesis by inhibiting chondrogenesis. J Cell Sci 124:2723-34 (2011).Read more (PubMed: 21807937) »
- Scotti Cet al. Recapitulation of endochondral bone formation using human adult mesenchymal stem cells as a paradigm for developmental engineering. Proc Natl Acad Sci U S A 107:7251-6 (2010). IHC-P; Human.Read more (PubMed: 20406908) »
See all 4 publications for this product
Publishing research using ab39012? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"