Overview

  • Product nameAnti-MMP14 antibody [114-6G6]
    See all MMP14 primary antibodies
  • Description
    Mouse monoclonal [114-6G6] to MMP14
  • Specificityab77965 does not react with mouse or rat. However, the sequence used as immunogen is identical in mouse and rat MMP14.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide:

    REVPYAYIREGHEK

    , corresponding to amino acids 160-173 of Human MMP14

  • EpitopeCatalytic domain.
  • Positive control
    • Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab77965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 20 µg/ml. Requires periodate-lysine-paraformaldehyde fixative for 18-24 hrs. at 4°C.
    WB: Use at a concentration of 8 - 20 µg/ml. Predicted molecular weight: 66 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSeems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
    • Tissue specificityExpressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      The precursor is cleaved by a furin endopeptidase.
    • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • Matrix metallopeptidase 14 (membrane inserted) antibody
      • Matrix metalloproteinase 14 antibody
      • Matrix metalloproteinase-14 antibody
      • Membrane type 1 matrix metalloproteinase antibody
      • Membrane type 1 metalloprotease antibody
      • Membrane type matrix metalloproteinase 1 antibody
      • Membrane-type matrix metalloproteinase 1 antibody
      • Membrane-type-1 matrix metalloproteinase antibody
      • MMP 14 antibody
      • MMP X1 antibody
      • MMP-14 antibody
      • MMP-X1 antibody
      • Mmp14 antibody
      • MMP14_HUMAN antibody
      • MMPX1 antibody
      • MT MMP 1 antibody
      • MT-MMP 1 antibody
      • MT1 MMP antibody
      • MT1-MMP antibody
      • MT1MMP antibody
      • MTMMP 1 antibody
      • MTMMP1 antibody
      see all

    Anti-MMP14 antibody [114-6G6] images

    • ab77965 at 20µg/ml staining MMP14 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue.

    References for Anti-MMP14 antibody [114-6G6] (ab77965)

    ab77965 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"