Overview

  • Product nameAnti-MMP14 antibody [3G4.2]
    See all MMP14 primary antibodies
  • Description
    Mouse monoclonal [3G4.2] to MMP14
  • Specificityab78290 recognizes the catalytic domain of MMP14, identifying the active form of the protein.
  • Tested applicationsICC, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein containing the catalytic domain of human MMP14

  • Positive control
    • MMP14 transfected U251 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab78290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB 1/200 - 1/2000. Predicted molecular weight: 66 kDa.

Target

  • FunctionSeems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • Tissue specificityExpressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrix metallopeptidase 14 (membrane inserted) antibody
    • Matrix metalloproteinase 14 antibody
    • Matrix metalloproteinase-14 antibody
    • Membrane type 1 matrix metalloproteinase antibody
    • Membrane type 1 metalloprotease antibody
    • Membrane type matrix metalloproteinase 1 antibody
    • Membrane-type matrix metalloproteinase 1 antibody
    • Membrane-type-1 matrix metalloproteinase antibody
    • MMP 14 antibody
    • MMP X1 antibody
    • MMP-14 antibody
    • MMP-X1 antibody
    • Mmp14 antibody
    • MMP14_HUMAN antibody
    • MMPX1 antibody
    • MT MMP 1 antibody
    • MT-MMP 1 antibody
    • MT1 MMP antibody
    • MT1-MMP antibody
    • MT1MMP antibody
    • MTMMP 1 antibody
    • MTMMP1 antibody
    see all

Anti-MMP14 antibody [3G4.2] images

  • Anti-MMP14 antibody [3G4.2] (ab78290) at 1/200 dilution + MMP14 transfected U251 cell lysate at 40 µg

    Predicted band size : 66 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-MMP14 antibody [3G4.2] (ab78290)

ab78290 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"