Overview

  • Product nameAnti-MMP14 antibody
    See all MMP14 primary antibodies
  • Description
    Rabbit polyclonal to MMP14
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, IHC-Fr, WB, ICC, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    REVPYAYIREGHEK

    , corresponding to amino acids 160-173 of Human MMP14

  • Positive control
    • Placenta, or Breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab3644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 19910495
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration. PubMed: 22002060

Target

  • FunctionSeems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • Tissue specificityExpressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrix metallopeptidase 14 (membrane inserted) antibody
    • Matrix metalloproteinase 14 antibody
    • Matrix metalloproteinase-14 antibody
    • Membrane type 1 matrix metalloproteinase antibody
    • Membrane type 1 metalloprotease antibody
    • Membrane type matrix metalloproteinase 1 antibody
    • Membrane-type matrix metalloproteinase 1 antibody
    • Membrane-type-1 matrix metalloproteinase antibody
    • MMP 14 antibody
    • MMP X1 antibody
    • MMP-14 antibody
    • MMP-X1 antibody
    • Mmp14 antibody
    • MMP14_HUMAN antibody
    • MMPX1 antibody
    • MT MMP 1 antibody
    • MT-MMP 1 antibody
    • MT1 MMP antibody
    • MT1-MMP antibody
    • MT1MMP antibody
    • MTMMP 1 antibody
    • MTMMP1 antibody
    see all

Anti-MMP14 antibody images

  • All lanes : Anti-MMP14 antibody (ab3644) at 1/500 dilution

    Lane 1 : 15ug MCF-7 whole cell lysate
    Lane 2 : 15ug MSTO-2H11 whole cell lysate
    Lane 3 : 15ug Malme-3M whole cell lysate
    Lane 4 : 15ug Calu-1 whole cell lysate
    Lane 5 : 15ug Sk-Lu-1 whole cell lysate

    Secondary
    Peroxidase-conjugated affinipure goat anti-rabbit
    developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 54,66 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa (possible non-specific binding),98 kDa (possible non-specific binding).

    Exposure time : 30 seconds

    See Abreview

  • ICC/IF image of ab3644 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3644, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab3644 staining in Hu Colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3644, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-MMP14 antibody (ab3644)

This product has been referenced in:
  • Lim HC  et al. Cell surface heparan sulfate proteoglycans control adhesion and invasion of breast carcinoma cells. Mol Cancer 14:15 (2015). Flow Cyt ; Human . Read more (PubMed: 25623282) »
  • Williams KC  et al. SNAP23, Syntaxin4, and vesicle-associated membrane protein 7 (VAMP7) mediate trafficking of membrane type 1-matrix metalloproteinase (MT1-MMP) during invadopodium formation and tumor cell invasion. Mol Biol Cell 25:2061-70 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24807903) »

See all 9 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Following-up my message from Friday, I did receive more information regarding ab3644: the immunogen is a 12 amino acid peptide found between amino acids 100-200 of human MPP-14. Since the pro-peptide is aa 1-111, and the antibody recognizes the active ...

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Thank you for your enquiry. I appreciate your continued patience in this matter. I have received some feedback from some of the sources of the antibodies that you are enquiring about; ab7033 - To follow on with the comments that I have made the...

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Thank you for your enquiry. Once again I am sorry to hear of the difficulties that you have experienced using these antibodies. I have had a look through the information that you have provided in conjunction with the blot images and I have a few co...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with these antibodies. The results that you have been obtaining are certainly troubling. What concerns me the most are the two bands that you are consistently det...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (cell lines: breast / lung)
Loading amount 15 µg
Specification cell lines: breast / lung
Blocking step Other as blocking agent for 30 minute(s) · Concentration: 0.05 % Twe
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Submitted May 04 2006

ab10800 -- Mouse monoclonal [HAS-3] to Integrin alpha 2 is provided at a concentration of 1mg/ml (this information was previosuly missing from the datasheet but has been added now). You do not mention which application the researcher plans to use the p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"