MMP14 antibody - Cytoplasmic domain (ab28209)

Product Name 

MMP14 antibody - Cytoplasmic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP14 - Cytoplasmic domain

Immunogen 

Synthetic peptide based on the Human MMP14.

Reacts with 
(species key)

Hu

Specificity 

This antibody is specific for MMP14, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc.).

We have a range of domain specific antibodies for this target. For a full list please see all MMP14 antibodies

Epitope 

This antibody binds to the cytoplasmic domain.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa). This antibody detects the pro form at 65kDa and the active form at 64kDa and also detects activation/breakdown products. The extracellular cleaved form is not detected. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

DU145 and HDFn cell lysate

Cellular localization 

Type I membrane protein

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc binding site characterizes the structure of the MMPs. In addition, fibronectin like repeats, a hinge region, and a C terminal hemopexin like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

MMP14 may be an activator of pro gelatinase A and is expressed in fibroblast cells during both wound healing and human cancer progression. MMP14 is expressed in very low levels and may require stimulation with concanavolin A or the phorbol ester TPA to stimulate production of MMP14.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

References for MMP14 antibody - Cytoplasmic domain (ab28209)

  This product has been used in:

Lafleur MA et al. Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell 20:2030-40 (2009). WB. PubMed: 19211836

If you publish research using ab28209 please let us know so that we can cite the reference on this datasheet.

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