Overview

  • Product nameAnti-MMP14 antibody [EP1264Y]
    See all MMP14 primary antibodies
  • Description
    Rabbit monoclonal [EP1264Y] to MMP14
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to residues in human MMP14.

  • Positive control
    • IHC: Human breast carcinoma tissue. WB: recombinant protein
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Alternative versions available:

    Anti-MMP14 antibody (Alexa Fluor® 488) [EP1264Y] (ab194240)

    Anti-MMP14 antibody (Alexa Fluor® 647) [EP1264Y] (ab194241)

    Anti-MMP14 antibody (HRP) [EP1264Y] (ab194242)

Properties

Applications

Our Abpromise guarantee covers the use of ab51074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/50 - 1/100.

Paraformaldehyde or methanol fixed cells.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/100.
WB 1/2000. Predicted molecular weight: 66 kDa.Can be blocked with MMP14 peptide (ab185123).

Target

  • FunctionSeems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • Tissue specificityExpressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrix metallopeptidase 14 (membrane inserted) antibody
    • Matrix metalloproteinase 14 antibody
    • Matrix metalloproteinase-14 antibody
    • Membrane type 1 matrix metalloproteinase antibody
    • Membrane type 1 metalloprotease antibody
    • Membrane type matrix metalloproteinase 1 antibody
    • Membrane-type matrix metalloproteinase 1 antibody
    • Membrane-type-1 matrix metalloproteinase antibody
    • MMP 14 antibody
    • MMP X1 antibody
    • MMP-14 antibody
    • MMP-X1 antibody
    • Mmp14 antibody
    • MMP14_HUMAN antibody
    • MMPX1 antibody
    • MT MMP 1 antibody
    • MT-MMP 1 antibody
    • MT1 MMP antibody
    • MT1-MMP antibody
    • MT1MMP antibody
    • MTMMP 1 antibody
    • MTMMP1 antibody
    see all

Anti-MMP14 antibody [EP1264Y] images

  • Immunofluorescence staining of MCF7 cells with purified ab51074 working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/2000 dilution + Human spleen lysate

    Secondary
    Goat anti-Rabbit HRP labeled. at 1/2000 dilution

    Predicted band size : 66 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HT1080 cells stained with ab51074 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51074, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG, H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • ab51074 at 1/500 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step (in Tris/EDTA) was performed. The tissue was then blocked with serum and incubated with the primary antibody. A biotinylated donkey anti-rabbit IgG was used as the secondary.

    See Abreview

  • Ab51074 (1:100) staining human MMP14 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

Protocols

References for Anti-MMP14 antibody [EP1264Y] (ab51074)

This product has been referenced in:
  • Zhao W  et al. Effect of TGF-ß1 on the Migration and Recruitment of Mesenchymal Stem Cells after Vascular Balloon Injury: Involvement of Matrix Metalloproteinase-14. Sci Rep 6:21176 (2016). WB, IHC-P ; Rat . Read more (PubMed: 26880204) »
  • Ding BS  et al. Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells. J Cell Sci 128:2983-8 (2015). WB ; Human . Read more (PubMed: 26116571) »

See all 28 Publications for this product

Product Wall

Thank you for contacting us.


According to our internal records, this immunogen isnot from the N-terminus. In addition, itwill recognise pro and mature forms.


I hope this information is helpful to you. Please do not hesi...

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Application Western blot
Sample Human Cell lysate - whole cell (Vascular smooth muscle cells)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification Vascular smooth muscle cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Oct 10 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Brain)
Permeabilization Yes - 0.1% Triton-X in PBS
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Mr. Daniel Lustberg

Verified customer

Submitted May 25 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Bos taurus Tissue sections (Airway)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Permeabilization No
Specification Airway
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 23 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue (frozen lung tissue)
Specification frozen lung tissue
Permeabilization Yes - triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 23 2014

We do not have any data for blocking studies with the rabbit monoclonal antibody ab51074. Currently, the only MMP14 antibody that we know has been tested for inhibition studies (assuming this is what you mean by "blocking") is the mouse monoclonal anti...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Heart, Left Ventricle)
Loading amount 40 µg
Specification Heart, Left Ventricle
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dawn Bowles

Verified customer

Submitted Dec 28 2012

Thank you for contacting us and your interest in our products. As discussed over the phone, I have checked the concentration of the antibodies and lot numbers you provided. The concentrations are as follows:

ab52897, GR5286-6, 0.165 mg/ml;
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Thank you for your enquiry.

I can confirm that the immunogen sequence for MMP14 antibody [EP1264Y] (ab51074) is derived from somewhere within amino acids 150-180 (extracellular region) of human MMP-14 which is in the extracellular domain. I ...

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Thank you for your enquiry.

I can confirm that ab51074 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on ...

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1-10 of 21 Abreviews or Q&A

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