Mouse, Rat, Human Predicted to work with:
Synthetic peptide corresponding to Human MMP14. (Peptide available as ab185123)
IHC: Human breast carcinoma, endometrium carcinoma and kidney tissues.
WB: Human fetal spleen, esophagus and lung cancer tissue lysates, mouse and rat spleen tissue lysates and A431 whole cell lysate.
Flow Cyt: A549 cells.
IP: A431 cell lysate.
A trial size is available to purchase for this antibody.
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
The precursor is cleaved by a furin endopeptidase.
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium carcinoma tissue sections labeling MMP14 with purified ab51074 at 1/100 dilution (1.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
All lanes : Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/5000 dilution (purified)
Lane 1 : Human fetal spleen lysate Lane 2 : Human lung cancer lysate Lane 3 : Mouse spleen lysate Lane 4 : Rat spleen lysate Lane 5 : Human esophagus lysate
Lysates/proteins at 20 µg per lane.
Secondary Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size : 66 kDa Observed band size : 66 kDa
Blocking/Diluting buffer and concentration 5% NFDM/TBST
63 kDa: pro-form; 60 kDa: active form.
MCF-7 is a MMP-14 negative or weakly expressed cell line (PMID: 25977338 and PMID: 19208838).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)This image is courtesy of an anonymous Abreview
ab51074 (unpurified) at 1/500 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step (in Tris/EDTA) was performed. The tissue was then blocked with serum and incubated with the primary antibody. A biotinylated donkey anti-rabbit IgG was used as the secondary.