|
|
|
|
|
Product Name
|
MMP15 antibody - Hinge region
|
| |
See all MMP15 antibodies (8)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to MMP15 - Hinge region
|
|
Immunogen
|
Synthetic peptide based on a sequence from Human MMP15.
(Peptide available as ab41094.)
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
The antibody binds to the hinge region of MMP15, and can recognize native or reduced MMP15. It does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9).
We have a range of domain specific antibodies for this target. For a full list please see all MMP15 antibodies
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 chemiluminescent substrates. When used against the reduced protein, the antibody identifies bands at 72 kDa and 55 kDa as well as activation/breakdown product at 48 kDa. Often the MMP15 is fully activated, and the 48 kDa band is the dominant band seen. Predicted molecular weight: 76 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Cell media from human chondrosarcoma.
|
|
Cellular localization
|
Membrane; single pass type I membrane protein.
|
|
Research areas
|
Cancer >> Tumor biomarkers >> Enzymes >> MMPs Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
|
|
Relevance
|
The mammalian matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT MMPs exist as integral membrane proteins. MMP15, first described as MTMMP2, is a metalloproteinase with a type I transmembrane domain. Initially reported in lung tissue, MMP15 message was also seen in testis, intestines, colon, heart, liver, kidney, pancreas and muscle. Western blots showed bands at 72 kD, 48 kD and 44 kD. Subsequent publications have focused on message, and found MMP15 in a wide range of tumor cell lines, as well as the synovial membrane from patients with rheumatoid arthritis. MMP15 is usually found in much lower levels than the more ubiquitous MMP14 (MT1MMP). MMP15 may be shed into the culture media when activated, but it is unclear if the shed form is proteolytically active. Cell lysates are the best source for Western blotting of MMP15.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: Sodium Azide Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product: Glycerol MSDS Sodium Azide MSDS
|
|
Purification notes
|
The antibody has been peptide affinity purified.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP15 antibody - Hinge region is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
|
Search PubMed (MEDLINE) for references to MMP15
|
| |
MMP15 antibody - Hinge region - more information
|
Customer reviews (feedback) regarding MMP15 antibody - Hinge region |
Customer FAQs regarding MMP15 antibody - Hinge region |
Protocols for MMP15 antibody - Hinge region |
Price and availability of products related to MMP15 antibody - Hinge region |
| |
MMP15 antibody - Hinge region - related products:
|
|
|
| |
MMP15 antibody - Hinge region - other tools:
|
Contact Abcam with a Technical Enquiry about MMP15 antibody - Hinge region |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"