MMP15 antibody - Hinge region (ab39025)

Product Name 

MMP15 antibody - Hinge region

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP15 - Hinge region

Immunogen 

Synthetic peptide based on a sequence from Human MMP15.

(Peptide available as ab41094.)

Reacts with 
(species key)

Hu

Specificity 

The antibody binds to the hinge region of MMP15, and can recognize native or reduced MMP15. It does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9).

We have a range of domain specific antibodies for this target. For a full list please see all MMP15 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 chemiluminescent substrates. When used against the reduced protein, the antibody identifies bands at 72 kDa and 55 kDa as well as activation/breakdown product at 48 kDa. Often the MMP15 is fully activated, and the 48 kDa band is the dominant band seen. Predicted molecular weight: 76 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Cell media from human chondrosarcoma.

Cellular localization 

Membrane; single pass type I membrane protein.

Research areas 

Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

The mammalian matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT MMPs exist as integral membrane proteins.
MMP15, first described as MTMMP2, is a metalloproteinase with a type I transmembrane domain. Initially reported in lung tissue, MMP15 message was also seen in testis, intestines, colon, heart, liver, kidney, pancreas and muscle. Western blots showed bands at 72 kD, 48 kD and 44 kD. Subsequent publications have focused on message, and found MMP15 in a wide range of tumor cell lines, as well as the synovial membrane from patients with rheumatoid arthritis. MMP15 is usually found in much lower levels than the more ubiquitous MMP14 (MT1MMP). MMP15 may be shed into the culture media when activated, but it is unclear if the shed form is proteolytically active. Cell lysates are the best source for Western blotting of MMP15.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody has been peptide affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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