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Read our guarantee »Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Anti-MMP15 antibody - Hinge region
See all MMP15 products (8) ...
Rabbit polyclonal to MMP15 - Hinge region
The antibody binds to the hinge region of MMP15, and can recognize native or reduced MMP15. It does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9).
Reacts with
Human
Synthetic peptide based on a sequence from Human MMP15.
(Peptide available as ab41094.)
Cell media from human chondrosarcoma.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration information loading...
Immunogen affinity purified
The antibody has been peptide affinity purified.
Polyclonal
IgG
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Our Abpromise guarantee covers the use of ab39025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 chemiluminescent substrates. When used against the reduced protein, the antibody identifies bands at 72 kDa and 55 kDa as well as activation/breakdown product at 48 kDa. Often the MMP15 is fully activated, and the 48 kDa band is the dominant band seen. Predicted molecular weight: 76 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.
Appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle.
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
The precursor is cleaved by a furin endopeptidase.
Membrane.
Target information above from: UniProt accessionP51511
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
ab39025 has not yet been referenced specifically in any publications.
Publishing research using ab39025? Please let us know so that we can cite the reference in this datasheet
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