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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP15 antibody - Hinge region
See all MMP15 products (8) ...
Description
Rabbit polyclonal to MMP15 - Hinge region
Specificity
The antibody binds to the hinge region of MMP15, and can recognize native or reduced MMP15. It does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on a sequence from Human MMP15.
(Peptide available as ab41094.)
Positive control
Cell media from human chondrosarcoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 chemiluminescent substrates. When used against the reduced protein, the antibody identifies bands at 72 kDa and 55 kDa as well as activation/breakdown product at 48 kDa. Often the MMP15 is fully activated, and the 48 kDa band is the dominant band seen. Predicted molecular weight: 76 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.
Tissue specificity
Appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Membrane.
Target information above from: UniProt accessionP51511
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MMP 15 antibody
- Matrix metallopeptidase 15 membrane inserted antibody
- Matrix metalloproteinase 15 antibody
see all
Database links
- Entrez Gene: 4324 Human
- Omim: 602261 Human
- SwissProt: P51511 Human
- Unigene: 80343 Human
Protocols
References for Anti-MMP15 antibody - Hinge region (ab39025)
ab39025 has not yet been referenced specifically in any publications.
Publishing research using ab39025? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"