Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)
Key features and details
- Mouse monoclonal [MM0028-5D11] to MT2-MMP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MT2-MMP antibody [MM0028-5D11]
See all MT2-MMP primary antibodies -
Description
Mouse monoclonal [MM0028-5D11] to MT2-MMP -
Host species
Mouse -
Specificity
ab56308 recognizes human MT2-MMP in western blot without cross reactivity with human MMP14/16. Reactivity with MT2-MMP in other species has not been tested. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human MT2-MMP.
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Positive control
- WB: Human placenta tissue lysate. IHC-P: Human placenta tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MM0028-5D11 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab56308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/400 - 1/1000. Predicted molecular weight: 76 kDa.
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IHC-P |
1/50 - 1/200.
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Notes |
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WB
1/400 - 1/1000. Predicted molecular weight: 76 kDa. |
IHC-P
1/50 - 1/200. |
Target
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Function
Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A. -
Tissue specificity
Appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4324 Human
- Omim: 602261 Human
- SwissProt: P51511 Human
- Unigene: 80343 Human
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Alternative names
- AI503551 antibody
- Matrix metallopeptidase 15 membrane inserted antibody
- Matrix metalloproteinase 15 antibody
see all
Images
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Anti-MT2-MMP antibody [MM0028-5D11] (ab56308) at 1/400 dilution + Human placenta tissue lysate
Predicted band size: 76 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted? -
ab56308 at 1/200 dilution, staining MT2-MMP in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Datasheets and documents
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Datasheet download
References (1)
ab56308 has been referenced in 1 publication.
- Yi G et al. A study of the mechanism of small-molecule soybean-protein-derived peptide supplement to promote sleep in a mouse model. RSC Adv 10:11264-11273 (2020). PubMed: 35495343