MMP16 antibody - Cytoplasmic domain (ab38967)

Product Name 

MMP16 antibody - Cytoplasmic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP16 - Cytoplasmic domain

Immunogen 

Synthetic peptide based on the cytoplasmic domain of human MMP16.

Reacts with 
(species key)

Hu

Specificity 

Does not cross react with other MTMMP or MMP family members (MMP-14, MMP-15, MMP-17, MMP1, MMP2, MMP3, MMP-9, etc).

We have a range of domain specific antibodies for this target. For a full list please see all MMP16 antibodies

Epitope 

This antibody binds to the Cytoplasmic tail of MMP16. For the antibody to gain access to the epitope, cells or tissue need to be permeabilized.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: A recommended starting concentration for Western blots is 1:2,000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. When used against the reduced protein, Ab38967 identifies bands at 65 Kd and 63 Kd (the pro-form and active form), as well as activation/breakdown products. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted protein; extracellular space; extracellular matrix

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane bound zinc endopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc binding site characterizes the structure of the MMPs. In addition, fibronectin like repeats, a hinge region, and a C terminal hemopexin like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

MMP16 induces the activation of pro gelatinase A (MMP2). It was identified as a membrane bound Metalloproteinase in normal and tumor cell lines. MMP16 is similar to the other MtMMPs; it contains a furin cleavage site, is membrane bound, and contains a cytoplasmic tail (MT4MMP lacks the tail, and may not be intercalated into the membrane). MMP16 is also known to be "shed" from the membrane in a soluble form. MT1MMP is known to function in activating a number of MMPs, chiefly MMP2, but that role has not been well described for the other MTMMPs. MMP16 has been reported to be elevated in several tumor cell lines, and is constituitively produced by some normal cell lines.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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