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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP16 antibody - Hinge region
See all MMP16 products (9) ...
Description
Rabbit polyclonal to MMP16 - Hinge region
Specificity
Ab38968 binds to MMP16, and can recognize the native and reduced forms. It does not cross react with the other MMP family members (MMP14, MMP15, MMP17, MMP1, MMP2, MMP3, MMP9, etc).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the hinge region of human MMP16.
Epitope
Ab38968 binds to the hinge region of MMP16.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Recommended starting dilution of 1/2000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentration of antibody may be needed for non human samples. When used against the reduced protein, ab38968 identifies bands at 65 kDa and 63 kDa (the pro form and active form respectively), as well as activation/breakdown products. Predicted molecular weight: 70 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
Tissue specificity
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Developmental stage
Expressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Cell membrane. Localized at the cell surface of melanoma cells and Secreted > extracellular space > extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells.
Target information above from: UniProt accessionP51512
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrix Metalloproteinase 16 antibody
- Matrix metalloproteinase-16 antibody
- Membrane type 3 matrix metalloproteinase antibody
see all
Database links
- Entrez Gene: 4325 Human
- Omim: 602262 Human
- SwissProt: P51512 Human
- Unigene: 492187 Human
- Unigene: 546267 Human
Protocols
References for Anti-MMP16 antibody - Hinge region (ab38968)
This product has been referenced in:
- Tatti Oet al. Membrane-Type-3 Matrix Metalloproteinase (MT3-MMP) Functions as a Matrix Composition-Dependent Effector of Melanoma Cell Invasion. PLoS One 6:e28325 (2011). WB, ICC/IF; Human.Read more (PubMed: 22164270) »
See 1 publication for this product
Publishing research using ab38968? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"