Overview

  • Product nameAnti-MMP17 antibody [EP1270Y]
    See all MMP17 primary antibodies
  • Description
    Rabbit monoclonal [EP1270Y] to MMP17
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-FoFrmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on human MMP17.

  • Positive control
    • Mouse brain lysate, human lymphoma tissue or HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab51075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 67 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22022509
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionEndopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.
    • Tissue specificityExpressed in brain, leukocytes, colon, ovary testis and breast cancer. Expressed also in many transformed and non-transformed cell types.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      The precursor is cleaved by a furin endopeptidase.
    • Cellular localizationCell membrane. Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Matrix metalloproteinase 17 antibody
      • Matrix metalloproteinase 17 membrane inserted antibody
      • Matrix metalloproteinase-17 antibody
      • Membrane type 4 matrix metalloproteinase antibody
      • Membrane type matrix metalloproteinase 4 antibody
      • Membrane-type matrix metalloproteinase 4 antibody
      • Membrane-type-4 matrix metalloproteinase antibody
      • MMP 17 antibody
      • MMP-17 antibody
      • Mmp17 antibody
      • MMP17_HUMAN antibody
      • MT MMP 4 antibody
      • MT MMP4 antibody
      • MT-MMP 4 antibody
      • MT4 MMP antibody
      • MT4-MMP antibody
      • MT4MMP antibody
      • MTMMP 4 antibody
      • MTMMP4 antibody
      see all

    Anti-MMP17 antibody [EP1270Y] images

    • Anti-MMP17 antibody [EP1270Y] (ab51075) at 1/1000 dilution + Mouse brain lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 67 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    • Ab51075 (1:50) staining human MMP17 in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunofluorescent staining of HeLa cells using ab51075 (1:50).
    • Immunohistochemical analysis of rat basolateral amygdala tissue, staining MMP17 with ab51075.

      Tissue was permeabilized with 0.5% Triton-X 100 before incubating with primary antibody (1/100) for 48 hours at 4°C. An AlexaFluor®568 goat anti-rabbit IgG was used as the secondary antibody.

    References for Anti-MMP17 antibody [EP1270Y] (ab51075)

    This product has been referenced in:
    • Sohail A  et al. Characterization of the Dimerization Interface of Membrane Type 4 (MT4)-Matrix Metalloproteinase. J Biol Chem 286:33178-89 (2011). WB ; Mouse . Read more (PubMed: 21828052) »
    • Li C  et al. Synergistic Activation of Dopamine D1 and TrkB Receptors Mediate Gain Control of Synaptic Plasticity in the Basolateral Amygdala. PLoS One 6:e26065 (2011). IHC-FoFr ; Rat . Read more (PubMed: 22022509) »

    See all 4 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (human cell lines)
    Specification human cell lines
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Mrs. Monika Schumacher

    Verified customer

    Submitted Feb 25 2015

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10 %)
    Sample Guinea pig Tissue lysate - whole (Liver)
    Specification Liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Mrs. Monika Schumacher

    Verified customer

    Submitted Jan 23 2015

    r these details. I am sorry this vial of antibody has not worked for this customer.

    As requested, we are pleased to arrange an alternative free of charge replacement of ab39028. I can confirm that 1 vial of ab39028 has been added to your ord...

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    Thank you for your message and for providing this further information.

    I am sorry there are no further suggestions to help improve the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased t...

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    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will provide us with vital informa...

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    Thank you for your inquiry.

    The concentration of ab51075 is lot dependent. The lot that we have in stock at the moment and of the last vial you receivedhas a concentration of 0.224 mg/mL.

    I also would like to comment on the seemingl...

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    Vielen Dank für Ihren Anruf gestern.

    Ich habe jetzt genauere Information zum Immunogen erhalten:

    Es liegt zwischen den Aminosäuren AA190 und 230 (SwissProt ID Q9ULZ9).

    Ich hoffe, diese Information hilft Ihnen her...

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    Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instr...

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    Thank you for your inquiry. I am happy to confirm that the concentration of lot GR19885 is 0.224 mg/mL. I also would like to comment on the seemingly low concentration: The IgG produced by rabbits have greater affinity and greater sensitivity ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"