| | | | Immunocytochemistry/ Immunofluorescence-MMP19 antibody - Aminoterminal end(ab39002)
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| Product Name | MMP19 antibody - Aminoterminal end |
| | See all MMP19 antibodies (9)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to MMP19 - Aminoterminal end |
| Immunogen | Synthetic peptide based on the aminoterminal end of active human MMP19.
(Peptide available as ab41108.) |
| Reacts with
(species key) | Hu |
| Specificity | ab39002 recognises the Aminoterminal end active MMP19. It does not cross react with the other MMP family members. We have a range of domain specific antibodies for this target. For a full list please see all MMP19 antibodies |
| Tested applications
(see key) | WB |
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| Application notes
(see key) | Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 57 kDa. When used against the reduced protein, ab39002 identifies bands at 58 Kd, 45 Kd, and a series of further cleaved active forms. Note: the conditioned media may need to be concentrated for Western blot. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | Secreted |
| Research areas | Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP |
| Relevance | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP19 is one of the more recently identified MMPs, has several novel structural features, and so does not fall into the traditional MMP groupings. It is without a furin cleavage site or transmembrane domain and unlike the other MMPs, MMP19 has an 8-residue acidic patch at the beginning of the hinge region. MMP19 is not constituitively produced by most cell types; it has been found in lung fibroblasts, and gingival fibroblasts when stimulated with TPA, as well as breast tumor and osteosarcoma cell lines. MMP19 may play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigens to anti-MMP19 are frequent in RA patients. |
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Purification notes | The antibody has been peptide-affinity purified. |
| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP19 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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| See below for MMP19 antibody - Aminoterminal end images, references, products related to ab39002 and other tools. |
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MMP19 antibody - Aminoterminal end images: |
 Immunocytochemistry/ Immunofluorescence-MMP19 antibody - Aminoterminal end(ab39002)
ICC/IF image of ab39002 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39002, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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| Search PubMed (MEDLINE) for references to MMP19 |
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