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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MMP19 antibody - Hinge region(ab38966)
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Product Name
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MMP19 antibody - Hinge region
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See all MMP19 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MMP19 - Hinge region
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Immunogen
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Synthetic peptide corresponding to the hinge region of human MMP19.
(Peptide available as ab41107.)
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Reacts with
(species key)
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Hu
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Specificity
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This antibody binds to the Hinge region of MMP19, to the reduced and non-reduced protein, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc.).
We have a range of domain specific antibodies for this target. For a full list please see all MMP19 antibodies
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for non-human samples. When used against the reduced protein, Ab38966 identifies bands at 58 Kd, 45 Kd, and a series of further cleaved active forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Relevance
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Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP19 is one of the more recently identified MMPs, has several novel structural features, and so does not fall into the traditional MMP groupings. It is without a furin cleavage site or transmembrane domain and unlike the other MMPs, MMP19 has an 8-residue acidic patch at the beginning of the hinge region. MMP19 is not constituitively produced by most cell types; it has been found in lung fibroblasts, and gingival fibroblasts when stimulated with TPA, as well as breast tumor and osteosarcoma cell lines. MMP19 may play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigens to anti-MMP19 are frequent in RA patients.
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MMP19 antibody - Hinge region (ab38966) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP19 antibody - Hinge region is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MMP19 antibody - Hinge region images, references, products related to ab38966 and other tools.
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MMP19 antibody - Hinge region images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MMP19 antibody - Hinge region(ab38966)
Ab38966 staining Human normal Liver parenchyma. Staining is localized to the cytoplasm and secreted.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Search PubMed (MEDLINE) for references to MMP19
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