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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP19 antibody - Hinge region
See all MMP19 products (10) ...
Description
Rabbit polyclonal to MMP19 - Hinge region
Specificity
This antibody binds to the Hinge region of MMP19, to the reduced and non-reduced protein, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc.).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to the hinge region of human MMP19.
(Peptide available as ab41107.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MMP19 antibody - Hinge region(ab38966)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for non-human samples. When used against the reduced protein, Ab38966 identifies bands at 58 Kd, 45 Kd, and a series of further cleaved active forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Endopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin.
Tissue specificity
Expressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients.
Involvement in disease
May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
Activated by autolytic cleavage after Lys-97.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ99542
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrix Metalloproteinase 16 antibody
- Matrix metalloproteinase 18 antibody
- Matrix metalloproteinase 19 antibody
see all
Database links
- Entrez Gene: 4327 Human
- Omim: 601807 Human
- SwissProt: C0M4B0 Human
- SwissProt: Q99542 Human
- Unigene: 154057 Human
- Unigene: 591033 Human
Anti-MMP19 antibody - Hinge region images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MMP19 antibody - Hinge region(ab38966)
Ab38966 staining Human normal Liver parenchyma. Staining is localized to the cytoplasm and secreted.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
References for Anti-MMP19 antibody - Hinge region (ab38966)
ab38966 has not yet been referenced specifically in any publications.
Publishing research using ab38966? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"