Overview

  • Product nameAnti-MMP2 antibody [4D3]
    See all MMP2 primary antibodies
  • Description
    Mouse monoclonal [4D3] to MMP2
  • SpecificityBinds to human MMP-2 and pro-MMP2. The antibody was tested for cross-reactivity with MMP-1, MMP-3, and MMP-9 and showed mild cross-reactivity with MMP-3.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human MMP2 aa 557-569 (internal sequence).
    Sequence:

    TSLGLPPDVQRVD


    Database link: P08253

  • Positive control
    • Colon carcinoma. Placental tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab2462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19910495
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 72 kDa gelatinase antibody
    • 72kD type IV collagenase antibody
    • CLG 4 antibody
    • CLG 4A antibody
    • CLG4 antibody
    • CLG4A antibody
    • Collagenase Type 4 alpha antibody
    • Collagenase type IV A antibody
    • Gelatinase A antibody
    • Gelatinase alpha antibody
    • Gelatinase neutrophil antibody
    • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
    • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 2 antibody
    • Matrix metalloproteinase II antibody
    • Matrix metalloproteinase-2 antibody
    • MMP 2 antibody
    • MMP II antibody
    • MMP-2 antibody
    • MMP2 antibody
    • MMP2_HUMAN antibody
    • MONA antibody
    • Neutrophil gelatinase antibody
    • PEX antibody
    • TBE 1 antibody
    • TBE-1 antibody
    see all

Anti-MMP2 antibody [4D3] images

  • 400 ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane, and detected by immunoblotting with ab2462 specific for MMP-2.

  • Anti-MMP2 antibody [4D3] (ab2462) at 1/5000 dilution + Rabbit aorta tissue lysate at 40 µg

    Secondary
    HRP-conjugate Sheep anti-mouse IgG polyclonal
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 72 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab2462 staining MMP2 in HT-1080 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were grown on glass coverslips, treated with PMA and then fixed with 4% (w/v) paraformaldehyde in phosphate buffered saline (PBS). Samples were then permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% (w/v) skimmed milk powder in PBS before staining with ab2462 and the appropriate secondary antibody, followed by washing and mounting.

    Scale bar: 10 µm.
  • IHC of human oesophageal adenocarcinoma labelling MMP2 with ab2462 (undiluted).  

References for Anti-MMP2 antibody [4D3] (ab2462)

This product has been referenced in:
  • Cui L  et al. Downregulation of B7-H4 in the MHCC97-H hepatocellular carcinoma cell line by arsenic trioxide. Mol Med Rep 13:2032-8 (2016). WB ; Human . Read more (PubMed: 26781180) »
  • Qi JS  et al. Combination of interventional adenovirus-p53 introduction and ultrasonic irradiation in the treatment of liver cancer. Oncol Lett 9:1297-1302 (2015). IHC ; Rabbit . Read more (PubMed: 25663901) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rabbit Tissue sections (skin)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: proteinase K
Permeabilization No
Specification skin
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative Formaldehyde
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Verified customer

Submitted Aug 11 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Permeabilization No
Specification skin
Blocking step human serum in 0.25% casein as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Acetone
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Verified customer

Submitted May 16 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Rabbit Tissue lysate - whole (aorta)
Specification aorta
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jun 24 2013

Thank you for confirming the quality of the secondary antibody. I can believe that the issue is with the MMP2 antibody but I am surprised that all three antibodies are giving you trouble.

Are the problems other people from other labs reporti...

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Thank you for the protocol details.


Do the blots for MMP1 and MMP9 look similar to the MMP2 blot? Can you please tell me the species of KB cells?


I am a little concerned aboutthe quality of the sample, given the multiple...

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Thank you for bringing this to our attention. Can you please describe your samples, how you prepare them for loading into the gel, how much antibody you use and how long you incubate, and any other details you think may be relevant? Are you confident t...

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Thank you for your enquiry.



Thank you for contacting us and sorry for the delay in getting back to you.



As discussed over the phone, unfortunately I can now confirm that the concentration of the Anti-MMP2 anti...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab2462.
To check the status of the order please contact our Cust...

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Thank you for your enquiry. We suggest a concentration of 1:20, however we also recommend a pilot experiment using a serial dilution with a range from 1:5 to 1:300 to determine the optimal concentration for the tissue you are staining. I hope t...

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Thank you for your enquiry. This Ab reacts with both the pro- and cleaved form of MMP2. Placental tissue can be used as a positive control. Should you have any further questions then please do not hesitate to get back in touch with me.

1-10 of 14 Abreviews or Q&A

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