Overview

  • Product nameAnti-MMP2 antibody [CA-4001]
    See all MMP2 primary antibodies
  • Description
    Mouse monoclonal [CA-4001] to MMP2
  • SpecificityThis antibody reacts with only the proform of MMP2 and shows no cross reaction with pro and active forms of MMP9 (Margulies et al 1992).
  • Tested applicationsSuitable for: Flow Cyt, WB, Inhibition Assaymore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Dog, Human
    Predicted to work with: Chicken, Cow, Pig
  • Immunogen

    N terminal peptide

    APSPIIKFPGDVAPKTDK

    of MMP2.

  • EpitopeN terminal (APSPIIKFPGD VAPKTDK)
  • Positive control
    • Conditioned, serum free medium from (TPA treated) HFL-1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab80737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 74 kDa. Incubate for 2 hours at room temperature.
Inhibition Assay Use a concentration of 2 - 4 µg/ml.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
      -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
      PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
    • Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
    • Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
      The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
    • Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
    • Information by UniProt
    • Database links
    • Alternative names
      • 72 kDa gelatinase antibody
      • 72kD type IV collagenase antibody
      • CLG 4 antibody
      • CLG 4A antibody
      • CLG4 antibody
      • CLG4A antibody
      • Collagenase Type 4 alpha antibody
      • Collagenase type IV A antibody
      • Gelatinase A antibody
      • Gelatinase alpha antibody
      • Gelatinase neutrophil antibody
      • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
      • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
      • Matrix Metalloproteinase 2 antibody
      • Matrix metalloproteinase II antibody
      • Matrix metalloproteinase-2 antibody
      • MMP 2 antibody
      • MMP II antibody
      • MMP-2 antibody
      • MMP2 antibody
      • MMP2_HUMAN antibody
      • MONA antibody
      • Neutrophil gelatinase antibody
      • PEX antibody
      • TBE 1 antibody
      • TBE-1 antibody
      see all

    Anti-MMP2 antibody [CA-4001] images

    • Overlay histogram showing MCF7 cells stained with ab80737 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80737, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.



    • Predicted band size : 74 kDa

      Western blot analysis of Human Cells-35 with ab80737

    References for Anti-MMP2 antibody [CA-4001] (ab80737)

    This product has been referenced in:
    • Liu GH  et al. MicroRNA-524-5p suppresses the growth and invasive abilities of gastric cancer cells. Oncol Lett 11:1926-1932 (2016). WB ; Human . Read more (PubMed: 26998102) »
    • Xi HQ  et al. Leucine-rich repeat-containing G-protein-coupled receptor 5 is associated with invasion, metastasis, and could be a potential therapeutic target in human gastric cancer. Br J Cancer 110:2011-20 (2014). Human . Read more (PubMed: 24594994) »

    See all 9 Publications for this product

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rabbit Tissue lysate - whole (Ligament)
    Loading amount 10 µg
    Specification Ligament
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Ms. Victoria Light

    Verified customer

    Submitted Jul 01 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Dog Tissue lysate - whole (Ligament)
    Loading amount 10 µg
    Specification Ligament
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Ms. Victoria Light

    Verified customer

    Submitted Jul 01 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"