Anti-MMP2 antibody [CA-4001 / CA719E3C] (ab3158)


  • Product nameAnti-MMP2 antibody [CA-4001 / CA719E3C]
    See all MMP2 primary antibodies
  • Description
    Mouse monoclonal [CA-4001 / CA719E3C] to MMP2
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 30-47 of MMP2.

  • EpitopeN-terminal (APSPIIKFPGD-VAPKTDK).
  • Positive control
    • Conditioned, serum-free medium from (TPA-treated) HFL-1 cells. Placenta.



Our Abpromise guarantee covers the use of ab3158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P 1/20.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 72 kDa (predicted molecular weight: 74 kDa). 1:200-1:400 for 2hrs at RT.


  • FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 72 kDa gelatinase antibody
    • 72kD type IV collagenase antibody
    • CLG 4 antibody
    • CLG 4A antibody
    • CLG4 antibody
    • CLG4A antibody
    • Collagenase Type 4 alpha antibody
    • Collagenase type IV A antibody
    • Gelatinase A antibody
    • Gelatinase alpha antibody
    • Gelatinase neutrophil antibody
    • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
    • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 2 antibody
    • Matrix metalloproteinase II antibody
    • Matrix metalloproteinase-2 antibody
    • MMP 2 antibody
    • MMP II antibody
    • MMP-2 antibody
    • MMP2 antibody
    • MMP2_HUMAN antibody
    • MONA antibody
    • Neutrophil gelatinase antibody
    • PEX antibody
    • TBE 1 antibody
    • TBE-1 antibody
    see all

Anti-MMP2 antibody [CA-4001 / CA719E3C] images

  • Anti-MMP2 antibody [CA-4001 / CA719E3C] (ab3158) at 1/50 dilution + human fetal lung cell-1 lysate

    Predicted band size : 74 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab3158 staining in Hu placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3158, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunohistochemical analysis of rat aorta tissue, staining MMP2 with ab3158 at 1/100 dilution. Staining was detected using DAB.
  • Overlay histogram showing MCF7 cells stained with ab3158 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3158, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-MMP2 antibody [CA-4001 / CA719E3C] (ab3158)

This product has been referenced in:
  • Bayoglu Tekin Y  et al. Is resveratrol a potential substitute for leuprolide acetate in experimental endometriosis? Eur J Obstet Gynecol Reprod Biol 184:1-6 (2015). IHC-P ; Rat . Read more (PubMed: 25462211) »
  • Wang J  et al. Qishenyiqi Dropping Pill attenuates myocardial fibrosis in rats by inhibiting RAAS-mediated arachidonic acid inflammation. J Ethnopharmacol 176:375-84 (2015). IHC ; Rat . Read more (PubMed: 26590099) »

See all 11 Publications for this product

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I have issued credit note number *** for the collagen and elastin antibodies. The purchase prices of these antibodies will be refunded back to your credit card pending approval f...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (osteosarcoma, skin with pyogranulamatous inflammat)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization No
Specification osteosarcoma, skin with pyogranulamatous inflammat
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Fixative Formaldehyde

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Submitted May 18 2015

I have issued a free of charge replacement order for ab51125.

You should recieve it tomorrow. To check the status of the order please contact our Customer Service team and reference this number.

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Thank you for sending the details of your samples and protocol. I think your protocol is good, though I do not know if MMP2 is expected to be expressed by prostate cancer cells.

In any case, we do not know what the band between 24 and 36 kDa...

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Thank you for bringing this to our attention. I do not know what the antibody is detecting in your sample, but I am assuming it is not related to MMP2, if you are reducing and denaturing your samples. Can you please tell me what the two samples were th...

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Thanks for keeping me updated abouttheprogress. I'm so glad to hear that ab7778 gave positive results!

Please let me know if there is anything else that you might need from me in the future. Have a wonderful weekend!

Thank you for your reply.

I am sorry to hear that both of these antibodies have not given good results. I don't have any notes about the elastin antibody ab21607, have you used it with the same protocol as ab6310? I would be happy to issue a ...

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Thank you for your phone call earlier this month and for letting us know about the trouble with these antibodies. We had discussed the IHC protocol that the lab used to test ab6310, and I have just received further information about this from the lab. ...

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I am very pleased to hear you would like to accept our offer and test ab3158 in mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiratio...

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Thank you for your call today and for our conversations about ab3158 and ab6310.

To reduce potential mouse-on-mous reaction with ab3158, I would recommend using an endogenous IgG blocking step with an anti-mouse F(ab) fragment such as ab6668-...

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