MMP2 antibody - Carboxyterminal end (ab38918)

Product Name 

MMP2 antibody - Carboxyterminal end

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP2 - Carboxyterminal end

Immunogen 

Synthetic peptide of Human MMP2 - Carboxyterminal end.

(Peptide available as ab41042.)

Reacts with 
(species key)

Hu

Specificity 

The antibody binds to Gelatinase A, but does not cross react with other MMP family members. This antibody recognizes reduced and native Gelatinase A, free or bound to TIMP2. It also recognizes the latent form of MMP2 as well as most of the activated forms. The antibody does not recognize the smallest active form of MMP2, since the hemopexin domain is cleaved off to produce this form. Gelatinase A is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen.

We have a range of domain specific antibodies for this target. For a full list please see all MMP2 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. The low protein levels produced (pg/ml) often require concentration of cell culture media to visualize the bands by Western Blotting. Gelatinase A and Gelatinase B may be enriched from conditioned cell culture media by binding to gelatin-agarose, and eluted by 10% DMSO. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Cell Media from human dermal fibroblasts neonatal, treated with IL1 beta.

Cellular localization 

Secreted

Research areas 

Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs

Relevance 

The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

MMP2, also known as Gelatinase A, is a type IV collagenase that specifically cleaves type IV collagen, the major structural component of basement membranes. The metastatic potential of tumor cells has been found to correlate with the activity of this enzyme.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: Sodium Azide
Constituents: 50% Glycerol, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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