MMP2 antibody - Propeptide domain (ab38919)

Product Name 

MMP2 antibody - Propeptide domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP2 - Propeptide domain

Immunogen 

Synthetic peptide corresponding to the Propeptide region of Human MMP2.

(Peptide available as ab41041.)

Reacts with 
(species key)

Hu

Specificity 

The antibody binds to the Propeptide domain of reduced and native MMP2, free or bound to TIMP2. The antibody recognizes only the latent form of MMP2, and thus can be used to distinguish the zymogen from the active enzyme. It does not cross react with the other MMP family members.

We have a range of domain specific antibodies for this target. For a full list please see all MMP2 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. MMP2 and MMP9 may be enriched from conditioned cell culture media by binding to gelatin-agarose, and eluted by 10% DMSO. The low protein levels produced (pg/ml) often require concentration of cell culture media to visualize the bands by Western Blotting. Detects a band of approximately 72 kDa (predicted molecular weight: 74 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

human MMP2, cell media from neonatal human dermal fibroblasts (IL1 beta treated).

Cellular localization 

Secreted

Research areas 

Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs

Relevance 

The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

MMP2, also known as Gelatinase A, is a type IV collagenase that specifically cleaves type IV collagen, the major structural component of basement membranes. The metastatic potential of tumor cells has been found to correlate with the activity of this enzyme.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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