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Anti-MMP2 antibody - Propeptide domain (ab38919)

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Overview

Product name

Anti-MMP2 antibody - Propeptide domain
See all MMP2 products (29) ...

Description

Rabbit polyclonal to MMP2 - Propeptide domain

Specificity

The antibody binds to the Propeptide domain of reduced and native MMP2, free or bound to TIMP2. The antibody recognizes only the latent form of MMP2, and thus can be used to distinguish the zymogen from the active enzyme. It does not cross react with the other MMP family members.

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide corresponding to the Propeptide region of Human MMP2.

(Peptide available as ab41041.)

Positive control

human MMP2, cell media from neonatal human dermal fibroblasts (IL1 beta treated).

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - MMP2 antibody - Propeptide domain (ab38919)Western blot - MMP2 antibody - Propeptide domain (ab38919) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab38919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. MMP2 and MMP9 may be enriched from conditioned cell culture media by binding to gelatin-agarose, and eluted by 10% DMSO. The low protein levels produced (pg/ml) often require concentration of cell culture media to visualize the bands by Western Blotting. Detects a band of approximately 72 kDa (predicted molecular weight: 74 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.

Tissue specificity

Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.

Involvement in disease

Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.

Sequence similarities

Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.

Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational
modifications

Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.

Cellular localization

Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.

Target information above from: UniProt accessionP08253 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 72 kDa gelatinase antibody
  • 72kD type IV collagenase antibody
  • CLG 4 antibody
  • CLG 4A antibody
  • CLG 4A antibody
  • CLG4 antibody
  • CLG4A antibody
  • CLG4A antibody
  • Collagenase Type 4 alpha antibody
  • Collagenase Type 4 alpha antibody
  • Collagenase type IV A antibody
  • Gelatinase A antibody
  • Gelatinase alpha antibody
  • Gelatinase alpha antibody
  • Gelatinase neutrophil antibody
  • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
  • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
  • Matrix Metalloproteinase 2 antibody
  • Matrix Metalloproteinase 2 antibody
  • Matrix metalloproteinase II antibody
  • Matrix metalloproteinase-2 antibody
  • MMP 2 antibody
  • MMP 2 antibody
  • MMP II antibody
  • MMP-2 antibody
  • MMP2 antibody
  • MMP2_HUMAN antibody
  • MONA antibody
  • Neutrophil gelatinase antibody
  • PEX antibody
  • TBE 1 antibody
  • TBE-1 antibody
see all

Anti-MMP2 antibody - Propeptide domain images:

  Western blot - MMP2 antibody - Propeptide domain (ab38919)

Western blot - MMP2 antibody - Propeptide domain (ab38919)

All lanes : Anti-MMP2 antibody - Propeptide domain (ab38919) at 1/1000 dilution

Lane 1 : Human MMP2
Lane 2 : Cell Media from human dermal fibroblasts (neonatal) treated with IL1 beta


Predicted band size : 74 kDa
Observed band size : 72 kDa (why is the actual band size different from the predicted?)

References for Anti-MMP2 antibody - Propeptide domain (ab38919)

ab38919 has not yet been referenced specifically in any publications.

Publishing research using ab38919? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"