Overview

  • Product nameAnti-MMP20 antibody
    See all MMP20 primary antibodies
  • Description
    Rabbit polyclonal to MMP20
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 396-445 (AAVYLREPQK TLFFVGDEYY SYDERKRKME KDYPKNTEEE FSGVNGQIDA) of Human MMP20 (NP_004762).

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab84737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionDegrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation.
  • Tissue specificityExpressed specifically in the enamel organ.
  • Involvement in diseaseDefects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2) [MIM:612529]. AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Autoactivates at least at the 107-Asn-
    -Tyr-108 site.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI2A2 antibody
    • Enamel metalloproteinase antibody
    • Enamelysin antibody
    • Matrix metalloproteinase 20 antibody
    • Matrix metalloproteinase-20 antibody
    • MMP 20 antibody
    • MMP-20 antibody
    • MMP20 antibody
    • MMP20_HUMAN antibody
    see all

Anti-MMP20 antibody images

  • Anti-MMP20 antibody (ab84737) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)

References for Anti-MMP20 antibody (ab84737)

This product has been referenced in:

See 1 Publication for this product

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