Recombinant
RabMAb

Anti-MMP20 antibody [EP1275Y] (ab76109)

Overview

  • Product name
    Anti-MMP20 antibody [EP1275Y]
    See all MMP20 primary antibodies
  • Description
    Rabbit monoclonal [EP1275Y] to MMP20
  • Tested applications
    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of human MMP20

  • Positive control
    • HeLa cell lysate; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation.
    • Tissue specificity
      Expressed specifically in the enamel organ.
    • Involvement in disease
      Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2) [MIM:612529]. AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.
    • Sequence similarities
      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • Domain
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      Autoactivates at least at the 107-Asn-
      -Tyr-108 site.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI2A2 antibody
      • Enamel metalloproteinase antibody
      • Enamelysin antibody
      • Matrix metalloproteinase 20 antibody
      • Matrix metalloproteinase-20 antibody
      • MMP 20 antibody
      • MMP-20 antibody
      • MMP20 antibody
      • MMP20_HUMAN antibody
      see all

    Anti-MMP20 antibody [EP1275Y] images

    • ab76109 at 1/100-1/250 dilution staining MMP20 in HeLa cells by Immunofluorescence.
    • Anti-MMP20 antibody [EP1275Y] (ab76109) at 1/500 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 54 kDa

    References for Anti-MMP20 antibody [EP1275Y] (ab76109)

    This product has been referenced in:
    • Yang T  et al. High amounts of fluoride induce apoptosis/cell death in matured ameloblast-like LS8 cells by downregulating Bcl-2. Arch Oral Biol 58:1165-73 (2013). WB ; Mouse . Read more (PubMed: 23598055) »
    • Mohazab L  et al. Critical role for avß6 integrin in enamel biomineralization. J Cell Sci 126:732-44 (2013). Read more (PubMed: 23264742) »

    See all 2 Publications for this product

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