Overview

  • Product name
  • Description
    Rabbit polyclonal to MMP27
  • Specificity
    ab75226 detects activated MMP27 resulting from cleavage adjacent to Tyr99.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: African green monkey
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide from human MMP27.

  • Positive control
    • COS-7 cells, treated with etoposide (25uM, 1hour).

Properties

Applications

Our Abpromise guarantee covers the use of ab75226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).
ELISA 1/10000.

Target

  • Function
    Matrix metalloproteinases degrade protein components of the extracellular matrix such as fibronectin, laminin, gelatins and/or collagens.
  • Tissue specificity
    Expressed in B-cells.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin repeats.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 3.4.24. antibody
    • Matrix metallopeptidase 27 antibody
    • Matrix metalloprotease 27 antibody
    • Matrix metalloproteinase 27 antibody
    • Matrix metalloproteinase-27 antibody
    • MMP 27 antibody
    • MMP-27 antibody
    • MMP27 antibody
    • MMP27_HUMAN antibody
    see all

Anti-MMP27 antibody images

  • All lanes : Anti-MMP27 antibody (ab75226) at 1/500 dilution

    Lane 1 : COS-7 cells, treated with etoposide (25uM, 1hour),
    Lane 2 : COS-7 cells, treated with etoposide (25uM, 1hour), with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 50 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 19 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MMP27 antibody (ab75226)

ab75226 has not yet been referenced specifically in any publications.

Product Wall

Thanks for your call today and for your questions about ab75226. Since this antibody has not been tested with rhesus samples, I can offer a discount off a future purchase if you buy ab75226 now, test it with rhesus samples and submit feedback to us in ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up