Overview

  • Product nameAnti-MMP27 antibody
    See all MMP27 primary antibodies
  • Description
    Rabbit polyclonal to MMP27
  • Specificityab75226 detects activated MMP27 resulting from cleavage adjacent to Tyr99.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: African Green Monkey
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide from human MMP27.

  • Positive control
    • COS-7 cells, treated with etoposide (25uM, 1hour).

Properties

Applications

Our Abpromise guarantee covers the use of ab75226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).
ELISA 1/10000.

Target

  • FunctionMatrix metalloproteinases degrade protein components of the extracellular matrix such as fibronectin, laminin, gelatins and/or collagens.
  • Tissue specificityExpressed in B-cells.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin repeats.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 3.4.24. antibody
    • Matrix metallopeptidase 27 antibody
    • Matrix metalloprotease 27 antibody
    • Matrix metalloproteinase 27 antibody
    • Matrix metalloproteinase-27 antibody
    • MMP 27 antibody
    • MMP-27 antibody
    • MMP27 antibody
    • MMP27_HUMAN antibody
    see all

Anti-MMP27 antibody images

  • All lanes : Anti-MMP27 antibody (ab75226) at 1/500 dilution

    Lane 1 : COS-7 cells, treated with etoposide (25uM, 1hour),
    Lane 2 : COS-7 cells, treated with etoposide (25uM, 1hour), with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 50 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 19 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MMP27 antibody (ab75226)

ab75226 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"