Anti-MMP28 antibody [EP1341(2)] (ab175937)
Key features and details
- Rabbit monoclonal [EP1341(2)] to MMP28
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-MMP28 antibody [EP1341(2)]
See all MMP28 primary antibodies -
Description
Rabbit monoclonal [EP1341(2)] to MMP28 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human placenta, human testis lysate, recombinant protein fragment
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1341(2) -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
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Related Buffer
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 62 kDa (predicted molecular weight: 59 kDa).
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 62 kDa (predicted molecular weight: 59 kDa). |
Target
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Function
Can degrade casein. Could play a role in tissues homeostasis and repair. -
Tissue specificity
Expressed at high levels in testes and lung. Low levels are detected in kidney, pancreas and skin. Also expressed in fetal lung, brain, skeletal muscle and kidney. Expressed selectively in keratinocytesis. Widely expressed in several carcinomas as well. Is up-regulated in response to injury in the skin. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 79148 Human
- Entrez Gene: 118453 Mouse
- Entrez Gene: 303384 Rat
- Omim: 608417 Human
- SwissProt: Q9H239 Human
- Unigene: 380710 Human
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Alternative names
- Epilysin antibody
- Matrix metalloproteinase 28 antibody
- Matrix metalloproteinase-28 antibody
see all
Images
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Anti-MMP28 antibody [EP1341(2)] (ab175937) at 1/1000 dilution + MMP-28 recombinant protein fragment at 0.01 µg
Predicted band size: 59 kDa -
All lanes : Anti-MMP28 antibody [EP1341(2)] (ab175937) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human testis lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 59 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab175937 has been referenced in 3 publications.
- Drury J et al. Upregulation of CD36, a Fatty Acid Translocase, Promotes Colorectal Cancer Metastasis by Increasing MMP28 and Decreasing E-Cadherin Expression. Cancers (Basel) 14:N/A (2022). PubMed: 35008415
- Botanská B et al. Matrix Metalloproteinases and Their Role in Mechanisms Underlying Effects of Quercetin on Heart Function in Aged Zucker Diabetic Fatty Rats. Int J Mol Sci 22:N/A (2021). PubMed: 33923282
- Su C et al. MiR-21 improves invasion and migration of drug-resistant lung adenocarcinoma cancer cell and transformation of EMT through targeting HBP1. Cancer Med 7:2485-2503 (2018). WB ; Human . PubMed: 29663730