The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at a concentration of 15 - 25 µg/ml.
IP: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 54 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Secreted > extracellular space > extracellular matrix.
Attia M et al. Early Events of Overused Supraspinatus Tendons Involve Matrix Metalloproteinases and EMMPRIN/CD147 in the Absence of Inflammation. Am J Sports Med41:908-17 (2013).
Read more (PubMed: 23404084) »
Clutterbuck AL et al. High throughput proteomic analysis of the secretome in an explant model of articular cartilage inflammation. J Proteomics74:704-15 (2011).
Read more (PubMed: 21354348) »