Overview

  • Product nameAnti-MMP3 antibodySee all MMP3 primary antibodies ...
  • Description
    Rabbit polyclonal to MMP3
  • Specificityab38916 recognises the Hinge region of MMP3. It does not cross react with the other Stromelysin forms (Stromelysin 2 and Stromelysin 3), nor to the other MMP family members (MMP1, MMP2, MMP9, etc). ab38916 does not recognize the 21 Kd active form.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the hinge region of human MMP3.

    (Peptide available as ab41045.)

  • Positive control
    • Cell medium from TPA treated human chondrosarcoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab38916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 54 kDa. But note that when used against the reduced protein, ab38916 identifies bands at 59 Kd and 57 Kd (the pro-form), as well as active forms. The antibody does not recognize the 21 Kd active form. Stromelysin levels in quiescent cells and tissues are minimal, and stimulation or protein concentration is often needed to visualize the bands. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase 3 preproprotein antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Progelatinase antibody
      • Proteoglycanase antibody
      • SL 1 antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelisin 1 antibody
      • Stromelysin 1 antibody
      • Stromelysin 1 progelatinase antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    Anti-MMP3 antibody images

    • All lanes : Anti-MMP3 antibody (ab38916) at 1/1000 dilution

      Lane 1 : human MMP3
      Lane 2 : cell medium from untreated human chondrosarcoma
      Lane 3 : cell medium from TPA treated human chondrosarcoma


      Performed under reducing conditions.

      Predicted band size : 54 kDa
      Observed band size : 45,57,59 kDa (why is the actual band size different from the predicted?)

    References for Anti-MMP3 antibody (ab38916)

    ab38916 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab38916.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"