Synthetic peptide derived from human MMP3 Peptide available as ab134501.
Our Abpromise guarantee covers the use of ab53015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
|WB||1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human MMP3 peptide (ab134501).|
|ICC/IF||Use a concentration of 5 µg/ml.|
Top image: Isotype control Middle image: Unstimulated cells Bottom image: IL-1 beta stimulated cells (24 hours, 10 ng/ml)
ab53015 staining MMP3 in Human synovial fibroblasts by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in methanol for 15 minutes at -20°C prior to blocking in 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 2 hours at 25°C. The secondary antibody was Cy5®-conjugated Goat anti-Rabbit polyclonal, diluted 1/200.