Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Secreted > extracellular space > extracellular matrix.
All lanes : Anti-MMP3 antibody (ab53015) at 1/500 dilution
Lane 1 : Extracts from 293 cells, untreated. Lane 2 : Extracts from 293 cells, treated with the immunising peptide.
Predicted band size: 54 kDa Observed band size: 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody (ab53015)This image is courtesy of an anonymous abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma labeling MMP3 with ab53015 at 1/100 dilution. Tissue was fixed with formaldehyde; heat-mediated antigen retrieval was performed using citrate buffer (pH 6.0). The tissue was blocked with 1% serum for 1 hour at 25°C. A biotin conjugated polyclonal goat anti-rabbit secondary antibody was used at 1/200 dilution.
ICC/IF image of ab53015 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53015, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using MMP3 antibody at a dilution of 1/50. Left: without immunizing peptide; Right: with immunizing peptide.
Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody (ab53015)This image is courtesy of an Abreview submitted by Torsten Lowin.
Top image: Isotype control Middle image: Unstimulated cells Bottom image: IL-1 beta stimulated cells (24 hours, 10 ng/ml)
ab53015 staining MMP3 in Human synovial fibroblasts by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in methanol for 15 minutes at -20°C prior to blocking in 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 2 hours at 25°C. The secondary antibody was Cy5®-conjugated Goat anti-Rabbit polyclonal, diluted 1/200.
Nordquist E et al. Postnatal and Adult Aortic Heart Valves Have Distinctive Transcriptional Profiles Associated With Valve Tissue Growth and Maintenance Respectively. Front Cardiovasc Med5:30 (2018).
Read more (PubMed: 29740591) »
Deng S et al. Local intra-articular injection of vascular endothelial growth factor accelerates articular cartilage degeneration in rat osteoarthritis model. Mol Med Rep17:6311-6318 (2018).
Read more (PubMed: 29488610) »