• Product nameAnti-MMP3 antibody [EP1186Y]See all MMP3 primary antibodies ...
  • Description
    Rabbit monoclonal [EP1186Y] to MMP3
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of human MMP3.

  • Positive control
    • Human gastric carcinoma tissue.
  • General notes

    Produced under U.S. Patent No. 5,675,063. A 40 μl trial size is available to purchase for this antibody.



Our Abpromise guarantee covers the use of ab52915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
IHC-P Use at an assay dependent dilution.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase 3 preproprotein antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Progelatinase antibody
      • Proteoglycanase antibody
      • SL 1 antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelisin 1 antibody
      • Stromelysin 1 antibody
      • Stromelysin 1 progelatinase antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    Anti-MMP3 antibody [EP1186Y] images

    • Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/1000 dilution + Recombinant protein (0.5ng)

      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • Ab52915 (1:100) staining human MMP3 in human gastric carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunohistochemical analysis of rat spinal tissue, staining MMP3 with ab52915.

      Sections were incubated with primary antibody in 2% BSA in PBS at a dilution of 1/250 overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.

    References for Anti-MMP3 antibody [EP1186Y] (ab52915)

    This product has been referenced in:
    • Tran CM  et al. CCN2 suppresses catabolic effects of interleukin-1ß through a5ß1 and aVß3 integrins in nucleus pulposus cells: implications in intervertebral disc degeneration. J Biol Chem 289:7374-87 (2014). WB . Read more (PubMed: 24464580) »
    • Ryu JH  et al. Hypoxia-inducible factor-2a is an essential catabolic regulator of inflammatory rheumatoid arthritis. PLoS Biol 12:e1001881 (2014). WB . Read more (PubMed: 24914685) »

    See all 8 Publications for this product

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