Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab52915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase 3 preproprotein antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Progelatinase antibody
      • Proteoglycanase antibody
      • SL 1 antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelisin 1 antibody
      • Stromelysin 1 antibody
      • Stromelysin 1 progelatinase antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    Anti-MMP3 antibody [EP1186Y] images

    • Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/1000 dilution + Recombinant protein (0.5ng)

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • Ab52915 (1:100) staining human MMP3 in human gastric carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunohistochemical analysis of rat spinal tissue, staining MMP3 with ab52915.

      Sections were incubated with primary antibody in 2% BSA in PBS at a dilution of 1/250 overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.

    References for Anti-MMP3 antibody [EP1186Y] (ab52915)

    This product has been referenced in:
    • Tran CM  et al. CCN2 suppresses catabolic effects of interleukin-1ß through a5ß1 and aVß3 integrins in nucleus pulposus cells: implications in intervertebral disc degeneration. J Biol Chem 289:7374-87 (2014). WB . Read more (PubMed: 24464580) »
    • Ryu JH  et al. Hypoxia-inducible factor-2a is an essential catabolic regulator of inflammatory rheumatoid arthritis. PLoS Biol 12:e1001881 (2014). WB . Read more (PubMed: 24914685) »

    See all 8 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Antigen retrieval step None
    Sample Human Tissue sections (cartilage)
    Specification cartilage
    Permeabilization No
    Fixative zinc buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Mar 19 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Antigen retrieval step None
    Sample Mouse Tissue sections (cartilage)
    Specification cartilage
    Permeabilization No
    Fixative zinc buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Mar 19 2015

    Thank you for contacting us.



    This product will detect total MMP3.



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    The MSDS can be downloaded from website. It is now linked to the datasheet.

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