Overview

  • Product nameAnti-MMP3 antibody [EP1186Y]
    See all MMP3 primary antibodies
  • Description
    Rabbit monoclonal [EP1186Y] to MMP3
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MMP3 aa 450 to the C-terminus (C terminal).

  • Positive control
    • Human gastric carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    A trial size is available to purchase for this antibody.

    Alternative versions available:

    Anti-MMP3 antibody (Alexa Fluor® 488) [EP1186Y] (ab194980)

    Anti-MMP3 antibody (Alexa Fluor® 647) [EP1186Y] (ab194717)

    Anti-MMP3 antibody (HRP) [EP1186Y] (ab194716)

Applications

Our Abpromise guarantee covers the use of ab52915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/50.
ICC/IF 1/100 - 1/250.

Target

  • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHDS6 antibody
    • Matrix metalloproteinase 3 antibody
    • Matrix metalloproteinase-3 antibody
    • MGC126102 antibody
    • MGC126103 antibody
    • MGC126104 antibody
    • MMP 3 antibody
    • MMP-3 antibody
    • MMP3 antibody
    • MMP3_HUMAN antibody
    • Proteoglycanase antibody
    • SL-1 antibody
    • SL1 antibody
    • STMY antibody
    • STMY1 antibody
    • STR1 antibody
    • Stromelysin 1 antibody
    • Stromelysin-1 antibody
    • Transin 1 antibody
    • Transin-1 antibody
    see all

Anti-MMP3 antibody [EP1186Y] images

  • Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/20000 dilution (purified) + MMP3 recombinant protein

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence staining of HT-29 cells with purified ab52915 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52915 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemical staining of paraffin embedded rat kidney with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded mouse liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/1000 dilution (Unpurified) + Recombinant protein (0.5ng)

    Secondary
    Goat anti-Rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Unpurified ab52915 (1:100) staining human MMP3 in human gastric carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

  • Immunohistochemical analysis of rat spinal tissue, staining MMP3 with ab52915.

    Sections were incubated with unpurified primary antibody in 2% BSA in PBS at a dilution of 1/250 overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.

References for Anti-MMP3 antibody [EP1186Y] (ab52915)

This product has been referenced in:
  • Zhao L  et al. Paired box 5 is a frequently methylated lung cancer tumour suppressor gene interfering ß-catenin signalling and GADD45G expression. J Cell Mol Med 20:842-54 (2016). WB ; Human . Read more (PubMed: 26843424) »
  • Peng C  et al. Norcantharidin Suppresses Colon Cancer Cell Epithelial-Mesenchymal Transition by Inhibiting the avß6-ERK-Ets1 Signaling Pathway. Sci Rep 6:20500 (2016). Read more (PubMed: 26846153) »

See all 13 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Antigen retrieval step None
Sample Human Tissue sections (cartilage)
Specification cartilage
Permeabilization No
Fixative zinc buffered formalin
Username

Abcam user community

Verified customer

Submitted Mar 19 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Antigen retrieval step None
Sample Mouse Tissue sections (cartilage)
Specification cartilage
Permeabilization No
Fixative zinc buffered formalin
Username

Abcam user community

Verified customer

Submitted Mar 19 2015

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This product will detect total MMP3.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

The MSDS can be downloaded from website. It is now linked to the datasheet.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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