Anti-MMP3 antibody [EP1186Y] (Alexa Fluor® 647) (ab194717)
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Overview
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Product nameAnti-MMP3 antibody [EP1186Y] (Alexa Fluor® 647)
See all MMP3 primary antibodies -
DescriptionRabbit monoclonal [EP1186Y] to MMP3 (Alexa Fluor® 647)
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Host speciesRabbit
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ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
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Tested applicationsSuitable for: ICC/IFmore details
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Species reactivityReacts with: Mouse
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MMP3 aa 450 to the C-terminus (C terminal).
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Positive control
- ICC/IF: NIH3T3 cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
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Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
PurityAffinity purified
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ClonalityMonoclonal
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Clone numberEP1186Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Related Products
Applications
Our Abpromise guarantee covers the use of ab194717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. |
Target
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FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
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Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 17392 Mouse
- SwissProt: P28862 Mouse
- Unigene: 4993 Mouse
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Alternative names
- CHDS6 antibody
- Matrix metalloproteinase 3 antibody
- Matrix metalloproteinase-3 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody [EP1186Y] (Alexa Fluor® 647) (ab194717)](http://a.abcam.com/ps/products/194/ab194717/Images/ab194717-237413-AP212881201.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody [EP1186Y] (Alexa Fluor® 647) (ab194717)ab194717 staining MMP3 in NIH3T3 cells. The cells were fixed with 100% formaldehyde (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab194717 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
References
ab194717 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"