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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP3 antibody - Hinge region
See all MMP3 products (22) ...
Description
Rabbit polyclonal to MMP3 - Hinge region
Specificity
ab38916 recognises the Hinge region of MMP3. It does not cross react with the other Stromelysin forms (Stromelysin 2 and Stromelysin 3), nor to the other MMP family members (MMP1, MMP2, MMP9, etc). ab38916 does not recognize the 21 Kd active form.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the hinge region of human MMP3.
(Peptide available as ab41045.)
Positive control
Cell medium from TPA treated human chondrosarcoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Western blot - MMP3 antibody (ab38916)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 54 kDa. But note that when used against the reduced protein, ab38916 identifies bands at 59 Kd and 57 Kd (the pro-form), as well as active forms. The antibody does not recognize the 21 Kd active form. Stromelysin levels in quiescent cells and tissues are minimal, and stimulation or protein concentration is often needed to visualize the bands. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP08254
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CHDS6 antibody
- Matrix metalloproteinase 3 antibody
- Matrix metalloproteinase 3 preproprotein antibody
see all
Database links
- Entrez Gene: 4314 Human
- Omim: 185250 Human
- SwissProt: P08254 Human
- Unigene: 375129 Human
Anti-MMP3 antibody - Hinge region images:
Western blot - MMP3 antibody (ab38916)
All lanes : Anti-MMP3 antibody - Hinge region (ab38916) at 1/1000 dilution
Lane 1 : human MMP3
Lane 2 : cell medium from untreated human chondrosarcoma
Lane 3 : cell medium from TPA treated human chondrosarcoma
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 45,57,59 kDa (why is the actual band size different from the predicted?)
The 59 and 57 kD bands represent MMP3 pro form, while the 45 kD band is the active form.
Protocols
References for Anti-MMP3 antibody - Hinge region (ab38916)
ab38916 has not yet been referenced specifically in any publications.
Publishing research using ab38916? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"