MMP3 antibody - Hinge region (ab38916)

Product Name 

MMP3 antibody - Hinge region

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP3 - Hinge region

Immunogen 

Synthetic peptide based on the hinge region of human MMP3.

(Peptide available as ab41045.)

Reacts with 
(species key)

Hu

Specificity 

ab38916 recognises the Hinge region of MMP3. It does not cross react with the other Stromelysin forms (Stromelysin 2 and Stromelysin 3), nor to the other MMP family members (MMP1, MMP2, MMP9, etc). ab38916 does not recognize the 21 Kd active form.

We have a range of domain specific antibodies for this target. For a full list please see all MMP3 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 54 kDa. But note that when used against the reduced protein, ab38916 identifies bands at 59 Kd and 57 Kd (the pro-form), as well as active forms. The antibody does not recognize the 21 Kd active form. Stromelysin levels in quiescent cells and tissues are minimal, and stimulation or protein concentration is often needed to visualize the bands. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Cell medium from TPA treated human chondrosarcoma.

Cellular localization 

Secreted; extracellular matrix

Research areas 

Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP3 is an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. MMP3 is reported to be implicated in rheumatoid arthritis and some forms of cancer.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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