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Abcam's MMP3 Inhibitor Screening Assay Kit (Colorimetric) (ab139439) is a complete assay system designed to screen MMP3 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5’-dithiobis(2-nitrobenzoic acid), Ellman’s reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (ε=13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format
This kit is useful to screen inhibitors of MMP3, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.
Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.
|Components||1 x 96 tests|
|96-well Clear Microplate (1/2 Volume)||1 unit|
|Colorimetric Assay Buffer||1 x 20ml|
|MMP Inhibitor||1 x 50µl|
|MMP Substrate||1 x 50µl|
|MMP3 Enzyme (Human, Recombinant)||1 x 30µl|
Our Abpromise guarantee covers the use of ab139439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab139439 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"