MMP3 Inhibitor Screening Assay Kit (Colorimetric) (ab139439)

Overview

  • Product name
    MMP3 Inhibitor Screening Assay Kit (Colorimetric)
    See all MMP3 kits
  • Detection method
    Colorimetric
  • Sample type
    Inhibitor compounds
  • Assay type
    Enzyme activity
  • Product overview

    Abcam's MMP3 Inhibitor Screening Assay Kit (Colorimetric) (ab139439) is a complete assay system designed to screen MMP3 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5’-dithiobis(2-nitrobenzoic acid), Ellman’s reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (ε=13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format

  • Notes

    This kit is useful to screen inhibitors of MMP3, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.

    Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab139439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Slope = V= 1.185E-02 OD/min

  • control slope = 1.4E-02 OD/min

    inhibitor slope = 1.3E-04 OD/min

    inhibitor % activity remaining = (1.3E-04/1.4E-02) x 100 = 0.9%

  • Km = 276 µM

    Vmax = 10.6 pmol/sec

Protocols

References

ab139439 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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