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Abcam MMP3 Inhibitor Screening Assay Kit (Fluorometric) (ab139440) is a complete assay system designed to screen MMP3 inhibitors using a quenched fluorogenic peptide: MMP Fluorogenic Substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 [Mca=(7-methoxycoumarin-4-yl)-acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α-β-diaminopropionyl]. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP3, a potential therapeutic target. The compound NNGH is also included as a prototypic control inhibitor.
|Components||1 x 96 tests|
|96-well White Microplate 1/2 Volume||1 unit|
|Fluorometric Assay Buffer||1 x 20ml|
|MMP Calibration Standard||1 x 50µl|
|MMP Fluorogenic Substrate||1 x 200µl|
|MMP Inhibitor||1 x 50µl|
|MMP3 Enzyme (Human, Recombinant)||1 x 30µl|
Our Abpromise guarantee covers the use of ab139440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab139440 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"