Overview

  • Product nameMMP3 Mouse ELISA KitSee all MMP3 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 117.4 106% - 130%
    Serum 120.6 101% - 140%
    Plasma 111.6 101% - 137%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s MMP3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MMP3 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse MMP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • Involvement in diseaseDefects in MMP3 are the cause of susceptibility to coronary heart disease type 6 (CHDS6) [MIM:614466]. A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries. Note=A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P08254 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • CHDS6
    • Matrix metalloproteinase 3
    • Matrix metalloproteinase 3 preproprotein
    • Matrix metalloproteinase-3
    • MGC126102
    • MGC126103
    • MGC126104
    • MMP 3
    • MMP-3
    • MMP3
    • MMP3_HUMAN
    • Progelatinase
    • Proteoglycanase
    • SL 1
    • SL-1
    • SL1
    • STMY
    • STMY1
    • STR1
    • Stromelisin 1
    • Stromelysin 1
    • Stromelysin 1 progelatinase
    • Stromelysin-1
    • Transin 1
    • Transin-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

MMP3 Mouse ELISA Kit images

  • Representative standard curve using ab100731

Protocols

References for MMP3 Mouse ELISA Kit (ab100731)

ab100731 has not yet been referenced specifically in any publications.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"